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PMID: 6320799 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The subcellular localization of ubiquinone in human neutrophils.

The Biochemical journal ·Vol. 216 ·No. 3 ·1983-12-15 ·Pages 765-8

Cross AR, Jones OT, Garcia R, Segal AW

Abstract

Ubiquinone-10 has recently been proposed as a component of the microbicidal oxidase system of neutrophil leukocytes [Crawford & Schneider (1982) J. Biol. Chem. 257, 6662-6668]. We have determined the subcellular localization of ubiquinone and could detect it only in the mitochondrial fractions. It was not enriched in phagolysosomes which were enriched in O2-. generating activity and cytochrome b-245. It is proposed that ubiquinone is unlikely to play a direct role in the electron transport chain located in the plasma membrane which functions to produce microbicidal oxygen radicals.

MeSH Terms
Centrifugation, Density Gradient Cytochrome b Group/blood Neutrophils/metabolism Subcellular Fractions/metabolism Superoxides/blood Ubiquinone/blood
Chemicals
Cytochrome b Group cytochrome b245 Superoxides Ubiquinone
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Cross A R
Jones O T
Garcia R
Segal A W
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21 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2160765
Published
1983-12-15
Pages
765-8
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1152573
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2160765 PMC Full Text View journal details
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