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PMID: 6320808 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Effects of vasopressin and corticosterone on fatty acid metabolism and on the activities of glycerol phosphate acyltransferase and phosphatidate phosphohydrolase in rat hepatocytes.

The Biochemical journal ·Vol. 217 ·No. 2 ·1984-01-15 ·Pages 461-9

Pollard AD, Brindley DN

Abstract

The effects of vasopressin on the short-term control of fatty acid metabolism were studied in isolated rat hepatocytes. Vasopressin increased the oxidation of oleate to CO2 and decreased the formation of ketones in hepatocytes from Wistar rats, but not from Brattleboro rats. Incubation with vasopressin for 30 min increased the conversion of oleate into triacylglycerol by 17% and 32% in hepatocytes from Wistar and Brattleboro rats respectively. The corresponding increases for the phospholipid fraction were 19% and 42%. When Wistar-rat hepatocytes were incubated with corticosterone for 6 h there was a 19% increase in triacylglycerol synthesis, and a 52% increase if vasopressin was added 30 min before the end of the incubation. Glycerol phosphate acyltransferase activity was not significantly increased by vasopressin. Incubation for 5-60 min with vasopressin increased the Vmax. of phosphatidate phosphohydrolase by 48% and 32% respectively in hepatocytes from Wistar and Brattleboro rats. These increases were antagonized if EGTA was added to the medium used for incubating the hepatocytes. The replacement of vasopressin by 5 microM-ionophore A23187 produced a significant increase of 13% in the phosphohydrolase activity. It is therefore likely that the effects of vasopressin on the phosphohydrolase are mediated by Ca2+. These results are discussed in relation to the possible function of phosphatidate phosphohydrolase in controlling the turnover of phosphoinositides, the synthesis of phosphatidylethanolamine, phosphatidylcholine and triacylglycerol, and the secretion of very-low-density lipoproteins.

MeSH Terms
Acyltransferases/metabolism Animals Arginine Vasopressin/pharmacology Calcimycin/pharmacology Calcium/pharmacology Corticosterone/pharmacology Egtazic Acid/pharmacology Fatty Acids/metabolism Glycerol-3-Phosphate O-Acyltransferase/metabolism In Vitro Techniques Liver/cytology,drug effects,metabolism Male Oleic Acid Oleic Acids/metabolism Phosphatidate Phosphatase/metabolism Phosphoric Monoester Hydrolases/metabolism Rats Rats, Brattleboro Rats, Inbred Strains
Chemicals
Fatty Acids Oleic Acids Arginine Vasopressin Oleic Acid Calcimycin Egtazic Acid Acyltransferases Glycerol-3-Phosphate O-Acyltransferase Phosphoric Monoester Hydrolases Phosphatidate Phosphatase Calcium Corticosterone
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pollard A D
Brindley D N
References (42)
42 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2170461
Published
1984-01-15
Pages
461-9
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1153237
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2170461 PMC Full Text View journal details
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