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PMID: 6321161 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

High efficiency of replication and expression of foreign genes in SV40-transformed human fibroblasts.

The EMBO journal ·Vol. 2 ·No. 12 ·1983-00-00 ·Pages 2327-31

Boast S, La Mantia G, Lania L, Blasi F

Abstract

Human fibroblasts (HF) were transformed in vitro with origin-defective SV40 DNA (ori-) using the calcium phosphate co-precipitation technique. The SV40 ori- transformed human cells (HSF) were able to replicate efficiently a recombinant DNA molecule containing the ori sequence of SV40 DNA. Transfection of HFS with pTBC1, a recombinant pi vx plasmid containing the herpes simplex virus thymidine kinase (HSV-TK) gene and the ori SV40 sequences, results in high levels of TK mRNA of correct size. The pTBC1 plasmid does not appear to contain 'poison' sequences and can be efficiently re-established in Escherichia coli after replication in human cells. This host vector system may be of great usefulness in studying the expression of human genes in human cells.

MeSH Terms
Animals Antigens, Viral, Tumor/genetics Base Sequence Cell Line Cell Transformation, Neoplastic DNA Replication DNA Restriction Enzymes DNA, Recombinant/metabolism Fibroblasts/metabolism Genes Genes, Viral Humans Plasmids Simian virus 40/genetics,immunology Simplexvirus/genetics Thymidine Kinase/genetics Transfection
Chemicals
Antigens, Viral, Tumor DNA, Recombinant Thymidine Kinase DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Boast S
La Mantia G
Lania L
Blasi F
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23 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1983-00-00
Pages
2327-31
Language
English
Region
England
NLM ID
8208664
PMCID
PMC555453
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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