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PMID: 6321448 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The dispersed, repeated family of MAL loci in Saccharomyces spp.

Journal of bacteriology ·Vol. 157 ·No. 3 ·1984-03-00 ·Pages 949-52

Michels CA, Needleman RB

Abstract

This report concludes our analysis of the various standard maltose-fermenting strains of Saccharomyces spp. We showed that, in addition to either the MAL2 or MAL4 functional locus present in the standard MAL2 and MAL4 strains, both strains contain two cryptic MALg genes mapping to the MAL1 and MAL3 locus positions. (Functional MAL loci appear to consist of two linked complementing gene functions, MALp and MALg. Cryptic MALg genes lack a linked functional MALp gene.) Using a probe containing a DNA fragment derived from the MAL6 locus, we detected three genomic HindIII fragments in both the MAL2 and MAL4 strains. Each of these HindIII fragments is shown to segregate in a Mendelian fashion and to be linked to one of the three MAL loci in each of the strains. We also detected additional fragments having significant sequence homology to the MAL6 probe but lacking in MAL-related functions.

MeSH Terms

Base Sequence Chromosome Mapping DNA Restriction Enzymes Deoxyribonuclease HindIII Fermentation Genes, Fungal Genetic Complementation Test Genetic Linkage Genotype Glucosidases/genetics Maltose/metabolism Saccharomyces/genetics,metabolism alpha-Glucosidases/genetics

Chemicals

Maltose DNA Restriction Enzymes Deoxyribonuclease HindIII Glucosidases alpha-Glucosidases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Michels C A

Needleman R B

References (12)

12 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.157.3.949-952.1984

Published

1984-03-00

Pages

949-52

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC215354

Subset

Grants

NIGMS NIH HHS · GM28216 · United States

NCRR NIH HHS · RR 07064 · United States

External Links

PubMed source DOI: 10.1128/jb.157.3.949-952.1984 PMC Full Text View journal details

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The dispersed, repeated family of MAL loci in Saccharomyces spp.

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