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PMID: 6321450 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Promoter-probe plasmid for Bacillus subtilis.

Journal of bacteriology ·Vol. 157 ·No. 3 ·1984-03-00 ·Pages 965-7

Donnelly CE, Sonenshein AL

Abstract

We have constructed a promoter-probe expression vector for Bacillus subtilis. This plasmid, pCED6, can be used to fuse various DNA sequences to the structural gene of Escherichia coli beta-galactosidase, permitting analysis of the promoter activity of such sequences. pCED6 replicates and confers drug resistances in both E. coli and B. subtilis.

MeSH Terms
Bacillus subtilis/genetics DNA Replication DNA Restriction Enzymes DNA, Recombinant Deoxyribonuclease HindIII Escherichia coli/genetics Genetic Vectors Operon Plasmids beta-Galactosidase/genetics
Chemicals
DNA, Recombinant DNA Restriction Enzymes Deoxyribonuclease HindIII beta-Galactosidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Donnelly C E
Sonenshein A L
References (8)
8 references, click to expand
  1. Cloning restriction fragments that promote expression of a gene in Bacillus subtilis.
    J Bacteriol. 1981 Jun;146(3):1162-5 PMID: 6787015
  2. Expression of Tn9-derived chloramphenicol resistance in Bacillus subtilis.
    Nature. 1981 Sep 24;293(5830):309-11 PMID: 6268988
  3. Identification of a new developmental locus in Bacillus subtilis by construction of a deletion mutation in a cloned gene under sporulation control.
    J Bacteriol. 1981 Oct;148(1):341-51 PMID: 6793556
  4. Analysis of gene control signals by DNA fusion and cloning in Escherichia coli.
    J Mol Biol. 1980 Apr;138(2):179-207 PMID: 6997493
  5. Use of a lacZ fusion to study the role of the spoO genes of Bacillus subtilis in developmental regulation.
    Cell. 1983 Nov;35(1):275-83 PMID: 6414720
  6. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.
    Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110-4 PMID: 4559594
  7. Characterization of plasmid transformation in Bacillus subtilis: kinetic properties and the effect of DNA conformation.
    Mol Gen Genet. 1979 Jan 2;167(3):251-8 PMID: 105246
  8. Chromogenic identification of genetic regulatory signals in Bacillus subtilis based on expression of a cloned Pseudomonas gene.
    Proc Natl Acad Sci U S A. 1983 Feb;80(4):1101-5 PMID: 6405380
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.157.3.965-967.1984
Published
1984-03-00
Pages
965-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215359
Subset
IM
Grants
NIGMS NIH HHS · R01-GM30408 · United States
External Links
PubMed source DOI: 10.1128/jb.157.3.965-967.1984 PMC Full Text View journal details
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