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PMID: 6322116 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Evolutionary conservation in the untranslated regions of actin mRNAs: DNA sequence of a human beta-actin cDNA.

Nucleic acids research ·Vol. 12 ·No. 3 ·1984-02-10 ·Pages 1687-96

Ponte P, Ng SY, Engel J, Gunning P, Kedes L

Abstract

We report the complete nucleotide sequence of a human beta actin cDNA. Both the 5' and 3' untranslated regions of the sequence are similar (greater than 80%) to the analogous regions of the rat beta-actin gene reported by Nudel et al (1983). When a segment of the 3' untranslated region is used as a radiolabelled probe, strong hybridization to chick beta actin mRNA is seen. This conservation of sequences suggests that strong selective pressures operate on non-translated segments of beta actin mRNA.

MeSH Terms
Actins/genetics Amino Acid Sequence Animals Base Sequence Biological Evolution Brain/metabolism Chickens Codon DNA/metabolism DNA Restriction Enzymes Genes Humans Liver/metabolism Muscles/metabolism Myocardium/metabolism Nucleic Acid Hybridization Protein Biosynthesis RNA, Messenger/genetics Rats Species Specificity
Chemicals
Actins Codon RNA, Messenger DNA DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Ponte P
Ng S Y
Engel J
Gunning P
Kedes L
References (14)
14 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.3.1687
Published
1984-02-10
Pages
1687-96
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318608
Subset
IM
Databases
GENBANK
J00074, M10278, X00351
External Links
PubMed source DOI: 10.1093/nar/12.3.1687 PMC Full Text View journal details
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