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PMID: 6322160 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Promoter-regulatory region of the major immediate early gene of human cytomegalovirus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 3 ·1984-02-00 ·Pages 659-63

Thomsen DR, Stenberg RM, Goins WF, Stinski MF

Abstract

The DNA templates containing immediate early (IE) genes of human cytomegalovirus (CMV) were transcribed in vitro by using a HeLa cell extract. When IE region 1, 2, and 3 were used, transcription was detected qualitatively only from IE region 1. Transcription was detected with DNA representing IE region 2 when the IE region 1 promoter was not present. DNA sequence analysis of the upstream regulatory region of IE region 1 detected two distinct repeats of 19 and 18 nucleotides, both being repeated four times. A putative cruciform structure could form through the surrounding sequences with each 18-nucleotide repeat being located in the unpaired region. The potential secondary structure and the repeat sequences in the regulatory region of IE region 1 are presumably related to the high level of transcription of this IE gene.

MeSH Terms
Base Sequence Cell Transformation, Viral Cytomegalovirus/genetics DNA Restriction Enzymes Genes Genes, Viral HeLa Cells/metabolism Humans Nucleic Acid Conformation Operon Plasmids Transcription, Genetic
Chemicals
DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Thomsen D R
Stenberg R M
Goins W F
Stinski M F
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29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.3.659
Published
1984-02-00
Pages
659-63
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC344894
Subset
IM
Grants
NIAID NIH HHS · AI100373 · United States
NIAID NIH HHS · AI13526 · United States
Databases
GENBANK
K01090, K01484
External Links
PubMed source DOI: 10.1073/pnas.81.3.659 PMC Full Text View journal details
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