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PMID: 6322996 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Calcium-dependent 4-aminopyridine stimulation of protein phosphorylation in squid optic lobe synaptosomes.

Cellular and molecular neurobiology ·Vol. 3 ·No. 3 ·1983-09-00 ·Pages 223-38

Pant HC, Gallant PE, Cohen R, Neary JT, Gainer H

Abstract

When intact synaptosomes were incubated with [gamma-32P]ATP, maximal protein phosphorylation was attained 2 min after the start of incubation. Protein phosphorylation under basal conditions was dependent on external Ca2+, and the dominant peak of phosphorylation was a 50-kd protein. Incubation of intact synaptosomes in the presence of 3-6 mM 4-aminopyridine (4-AP) caused a markedly enhanced phosphorylation of high molecular weight proteins of 90, 100, 130, and 180 kd, with no increase in the 50 or 38 kd proteins. This effect of 4-AP was dependent on external calcium ions in the incubation medium. The 4-AP effect on the high molecular weight proteins was also found in synaptosomal plasma membranes isolated from the synaptosomes. Tetraethylammonium (TEA) ions did not produce this enhancement of phosphorylation.

MeSH Terms
4-Aminopyridine Adenosine Triphosphate/metabolism Aminopyridines/pharmacology Animals Calcium/pharmacology Decapodiformes Electrophoresis, Polyacrylamide Gel Ethanolamines/pharmacology Ion Channels/drug effects Membrane Proteins/metabolism Microscopy, Electron Nerve Tissue Proteins/metabolism Optic Lobe, Nonmammalian/drug effects,metabolism Phosphorylation Synaptosomes/drug effects,metabolism
Chemicals
Aminopyridines Ethanolamines Ion Channels Membrane Proteins Nerve Tissue Proteins Adenosine Triphosphate triethanolamine 4-Aminopyridine Calcium
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Pant H C
Gallant P E
Cohen R
Neary J T
Gainer H
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27 references, click to expand
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Article Info
Journal
Cellular and molecular neurobiology
Abbr.
Cell Mol Neurobiol
ISSN
0272-4340
DOI
10.1007/BF00710949
Published
1983-09-00
Pages
223-38
Language
English
Region
United States
NLM ID
8200709
Subset
IM
Grants
NINDS NIH HHS · NS-15889 · United States
NINDS NIH HHS · NS-16610 · United States
External Links
PubMed source DOI: 10.1007/BF00710949 View journal details
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