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PMID: 6323736 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Subcellular distribution of viral structural proteins during simian virus 40 infection.

Journal of virology ·Vol. 50 ·No. 2 ·1984-05-00 ·Pages 363-71

Lin W, Hata T, Kasamatsu H

Abstract

The amounts of simian virus 40 structural polypeptides Vp1, Vp2, and Vp3 in different subcellular fractions at various times after lytic infection were determined by a quantitative immunoblotting procedure. Simian virus 40-infected cells were lysed with a buffer containing Nonidet P-40 to yield a soluble fraction. The Nonidet P-40-insoluble fraction was further fractionated in the presence of deoxycholate and Tween 40 to yield a soluble fraction (cytoskeletal) and an insoluble fraction (Nuc), which is primarily cell nuclei. At 33 h postinfection, the majority of viral structural proteins was found in the cell nucleus, whereas, at 48 to 65 h postinfection, Vp1 was distributed evenly among all cell fractions and Vp2 and Vp3 were found predominantly in the cytoskeletal and Nuc fractions. Thus, not all of the viral polypeptides synthesized in the cytoplasm migrated into the cell nucleus. Throughout infection, the molar ratio (Vp3/Vp2) was rather constant in all subcellular fractions, indicating that the synthesis or processing or both of Vp2 and Vp3 are coordinately regulated. The molar ratio of Vp1/(Vp2 + Vp3) varied among the fractions. The Vp1/(Vp2 + Vp3) molar ratio in the soluble fraction varied during the course of infection; however, constant ratios were maintained in the cytoskeletal and Nuc fractions. Thus, the mechanism which controls the movement of Vp1 to different compartments of the cell appears to be different from that of Vp2 and Vp3. The Vp1/(Vp2 + Vp3) value in the Nuc fraction was similar to the ratio found in virus particles. The constant molar distribution of Vp1, Vp2, and Vp3 in the Nuc fraction throughout infection suggests that there is a specific mechanism which regulates the transport of viral structural proteins. These results support the hypothesis that the structural proteins of simian virus 40 are transported into the cell nucleus in precise proportions.

MeSH Terms
Animals Antigen-Antibody Complex Cell Line Cell Transformation, Viral Chlorocebus aethiops Electrophoresis, Polyacrylamide Gel Immune Sera Kidney Kinetics Simian virus 40/genetics Subcellular Fractions/metabolism Viral Proteins/genetics,isolation & purification Viral Structural Proteins
Chemicals
Antigen-Antibody Complex Immune Sera Viral Proteins Viral Structural Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lin W
Hata T
Kasamatsu H
References (53)
53 references, click to expand
  1. On the electrotransfer of polypeptides from gels to nitrocellulose membranes.
    Anal Biochem. 1983 Feb 1;128(2):302-11 PMID: 6189419
  2. The genome of simian virus 40.
    Science. 1978 May 5;200(4341):494-502 PMID: 205947
  3. Complete nucleotide sequence of SV40 DNA.
    Nature. 1978 May 11;273(5658):113-20 PMID: 205802
  4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  5. Nuclear transplantation and the control of gene activity in animal development.
    Proc R Soc Lond B Biol Sci. 1970 Dec 1;176(1044):303-14 PMID: 4395100
  6. Cell-free translation of simian virus 40 16S and 19S L-strand-specific mRNA classes to simian virus 40 major VP-1 and minor VP-2 and VP-3 capsid proteins.
    J Virol. 1979 Mar;29(3):1204-12 PMID: 221674
  7. Host antigens in the centriolar region are induced in SV40-infected TC7 cells: SV40 small-T-function requirement.
    Cold Spring Harb Symp Quant Biol. 1980;44 Pt 1,:243-52 PMID: 6253138
  8. Structure of simian virus 40. II. Symmetry and components of the virus particle.
    Virology. 1967 Jul;32(3):511-23 PMID: 4291308
  9. Capsid proteins of Simian virus 40.
    Biochem Biophys Res Commun. 1970 Jul 13;40(1):97-102 PMID: 4318589
  10. Microinjection of the nonhistone chromosomal protein HMG1 into bovine fibroblasts and HeLa cells.
    Cell. 1979 Apr;16(4):901-8 PMID: 455454
  11. Altered protein metabolism in infection by the late tsB11 mutant of simian virus 40.
    J Virol. 1974 Oct;14(4):997-1007 PMID: 4371515
  12. The nuclear matrix: three-dimensional architecture and protein composition.
    Cell. 1982 Jul;29(3):847-58 PMID: 7151171
  13. A map of temperature-sensitive mutants of simian virus 40.
    Virology. 1975 Jul;66(1):70-81 PMID: 166506
  14. Messenger RNA is translated when associated with the cytoskeletal framework in normal and VSV-infected HeLa cells.
    Cell. 1981 Jan;23(1):113-20 PMID: 6260369
  15. Vp1 affects intracellular localization of Vp3 polypeptide during simian virus 40 infection.
    Proc Natl Acad Sci U S A. 1979 Jun;76(6):2808-12 PMID: 223160
  16. Deletion mutants of simian virus 40 generated by enzymatic excision of DNA segments from the viral genome.
    J Mol Biol. 1974 Oct 15;89(1):179-93 PMID: 4374552
  17. Direct biochemical mapping of eukaryotic viral DNA by means of a linked transcription-translation cell-free system.
    Proc Natl Acad Sci U S A. 1976 Aug;73(8):2747-51 PMID: 183208
  18. Protein metabolism in SV40-infected cells.
    Virology. 1973 Nov;56(1):313-33 PMID: 4355529
  19. Protein synthesis in Simian virus 40-infected monkey cells.
    Proc Natl Acad Sci U S A. 1972 Apr;69(4):921-4 PMID: 4337247
  20. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  21. Structure and function of the polypeptides in simian virus 40. I. Existence of subviral deoxynucleoprotein complexes.
    J Virol. 1972 Jun;9(6):923-9 PMID: 4113888
  22. A polypeptide domain that specifies migration of nucleoplasmin into the nucleus.
    Cell. 1982 Sep;30(2):449-58 PMID: 6814762
  23. Pattern of protein synthesis in monkey cells infected by simian virus 40.
    J Virol. 1972 May;9(5):758-65 PMID: 4337162
  24. Construction and analysis of viable deletion mutants of simian virus 40.
    J Virol. 1976 May;18(2):664-71 PMID: 178902
  25. Characterization of a temperature-sensitive, DNA-positive, nontransforming mutant of simian virus 40.
    Virology. 1981 Jul 30;112(2):605-24 PMID: 6266143
  26. Preparation of iodine-131 labelled human growth hormone of high specific activity.
    Nature. 1962 May 5;194:495-6 PMID: 14450081
  27. Intermediate filament systems are collapsed onto the nuclear surface after isolation of nuclei from tissue culture cells.
    Exp Cell Res. 1982 Mar;138(1):207-14 PMID: 6175528
  28. Synthesis and assembly of simian virus 40. II. Synthesis of the major capsid protein and its incorporation into viral particles.
    J Virol. 1972 Jan;9(1):52-60 PMID: 4333545
  29. A peptide comparison of proteins in the simian virus 40 virion.
    Virology. 1975 Dec;68(2):539-43 PMID: 173081
  30. Protein migration into nuclei. II. Frog oocyte nuclei accumulate a class of microinjected oocyte nuclear proteins and exclude a class of microinjected oocyte cytoplasmic proteins.
    J Cell Biol. 1975 Feb;64(2):431-7 PMID: 1117031
  31. Intracellular migration of nuclear proteins in Xenopus oocytes.
    Nature. 1978 Mar 16;272(5650):254-6 PMID: 564468
  32. Functional inactivation of bacteriophage lambda morphogenetic gene in RNA.
    Nature. 1975 Feb 20;253(5493):647-50 PMID: 1113861
  33. RNA metabolism in the HeLa cell nucleus.
    J Mol Biol. 1966 May;17(1):117-30 PMID: 5961144
  34. Structural polypeptides of simian virus 40.
    J Virol. 1971 May;7(5):635-41 PMID: 4326753
  35. Structural proteins of simian virus 40. I. Histone characteristics of low-molecular-weight polypeptides.
    J Virol. 1975 Feb;15(2):379-85 PMID: 163362
  36. Physical principles in the construction of regular viruses.
    Cold Spring Harb Symp Quant Biol. 1962;27:1-24 PMID: 14019094
  37. Resolution of simian virus 40 proteins in whole cell extracts by two-dimensional electrophoresis: heterogeneity of the major capsid protein.
    Cell. 1976 Oct;9(2):289-98 PMID: 184964
  38. Characterization of components released by alkali disruption of simian virus 40.
    J Virol. 1977 Mar;21(3):1079-84 PMID: 15134
  39. Resolutions and identification of the core deoxynucleoproteins of the simian virus 40.
    Biochem Biophys Res Commun. 1973 Sep 18;54(2):640-7 PMID: 4356978
  40. The B/C gene of simian virus 40.
    Virology. 1976 Dec;75(2):335-45 PMID: 64014
  41. Protein-SV40 DNA complex stable in high salt and sodium dodecyl sulfate.
    Biochem Biophys Res Commun. 1976 Feb 9;68(3):927-36 PMID: 177014
  42. Amino acid compositions of simian virus 40 structural proteins.
    Biochem Biophys Res Commun. 1973 Jun 19;52(4):1221-7 PMID: 4352238
  43. Evidence for post-transcriptional control of the morphogenetic genes of bacteriophage lambda.
    J Mol Biol. 1974 May 5;85(1):163-75 PMID: 4835731
  44. Biology of simian virus 40 (SV40) transplantation antigen (TrAg) II. Isolation and characterization of additional temperature-sensitive mutants of SV40.
    Virology. 1977 Sep;81(2):192-211 PMID: 197693
  45. An electron microscope study of the development of SV40 virus.
    J Cell Biol. 1963 May;17:423-41 PMID: 13963379
  46. Identification of virus-induced proteins in cells productively infected with simian virus 40.
    J Virol. 1972 Jan;9(1):1-9 PMID: 4333541
  47. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  48. Intracellular localization of viral polypeptides during simian virus 40 infection.
    J Virol. 1979 Nov;32(2):648-60 PMID: 228082
  49. Complementation analysis of simian virus 40 mutants.
    J Virol. 1974 May;13(5):1101-9 PMID: 4363253
  50. High resolution two-dimensional electrophoresis of proteins.
    J Biol Chem. 1975 May 25;250(10):4007-21 PMID: 236308
  51. Physical and genetic characterization of deletion mutants of simian virus 40 constructed in vitro.
    J Virol. 1977 Oct;24(1):277-94 PMID: 198579
  52. Protein migration into nuclei. I. Frog oocyte nuclei in vivo accumulate microinjected histones, allow entry to small proteins, and exclude large proteins.
    J Cell Biol. 1975 Feb;64(2):421-30 PMID: 46868
  53. Synthesis of the SV40 viral polypeptide Vp1 during infection.
    Virology. 1978 May 15;86(2):344-53 PMID: 208255
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.50.2.363-371.1984
Published
1984-05-00
Pages
363-71
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255629
Subset
IM
Grants
NCI NIH HHS · 5T32CA09030 · United States
NCRR NIH HHS · RR07009 · United States
External Links
PubMed source DOI: 10.1128/JVI.50.2.363-371.1984 PMC Full Text View journal details
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