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PMID: 6323957 Published · ppublish English Journal Article

Cyclic AMP-dependent regulation of activities of synthetase and phosphodiesterase of 2',5'-oligoadenylate in NIH 3T3 cells.

Molecular and cellular biochemistry ·Vol. 58 ·No. 1-2 ·1984-00-00 ·Pages 165-71

Itkes AV, Turpaev KT, Kartasheva ON, Kafiani CA, Severin ES

Abstract

Treatment of NIH 3T3 cells with adenylate cyclase activator adrenaline (10(-6) M) or cAMP phosphodiesterase inhibitor theophylline (10(-3) M) was shown to lead to intracellular cAMP elevation followed by a 2.0-to 2.5-fold increase in the 2',5'-oligoadenylate synthetase activity. This process was blocked by actinomycin D. The rise in the intracellular cAMP level was also followed by a 3-4-fold decrease in the activity of 2'-phosphodiesterase. Propranolol prevented this inhibition but actinomycin D produced only a negligible effect on the process. Incubation of the cell homogenate with purified catalytic subunit of cAMP-dependent protein kinase and ATP also resulted in a decrease of 2'-phosphodiesterase activity. These results indicate that cAMP is involved in the regulation of enzymes of the 2',5'-oligoadenylate system. The possibility that certain biological functions of cAMP are implemented via 2',5'-oligoadenylate-dependent processes is discussed.

MeSH Terms
2',5'-Oligoadenylate Synthetase/metabolism Adenine Nucleotides/metabolism Adenylyl Cyclases/metabolism Animals Cell Line Cyclic AMP/physiology Dactinomycin/pharmacology Epinephrine/pharmacology Exoribonucleases/metabolism Mice Oligonucleotides/metabolism Oligoribonucleotides/metabolism Phosphoric Diester Hydrolases/metabolism Protein Kinases/metabolism Theophylline/pharmacology
Chemicals
Adenine Nucleotides Oligonucleotides Oligoribonucleotides Dactinomycin 2',5'-oligoadenylate Theophylline Cyclic AMP Protein Kinases 2',5'-Oligoadenylate Synthetase Exoribonucleases 2'-phosphodiesterase Phosphoric Diester Hydrolases Adenylyl Cyclases Epinephrine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Itkes A V
Turpaev K T
Kartasheva O N
Kafiani C A
Severin E S
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33 references, click to expand
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Article Info
Journal
Molecular and cellular biochemistry
Abbr.
Mol Cell Biochem
ISSN
0300-8177
DOI
10.1007/BF00240616
Published
1984-00-00
Pages
165-71
Language
English
Region
Netherlands
NLM ID
0364456
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00240616 View journal details
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