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PMID: 6324084 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The avian malaria Plasmodium lophurae has a small number of heterogeneous ribosomal RNA genes.

Nucleic acids research ·Vol. 11 ·No. 23 ·1983-12-10 ·Pages 8443-59

Unnasch TR, Wirth DF

Abstract

The structure and number of the ribosomal RNA (rRNA) genes of the avian malaria parasite Plasmodium lophurae has been examined using Southern blot analysis and recombinant DNA techniques. The ribosomal DNA (rDNA) of P. lophurae has been cloned into the plasmid pBR322, beginning with size-selected populations of Cla I- and Hind III-restricted parasite DNA. The structure of two clones (CL-1 and HA-2) is presented in detail. These two clones together probably represent the entire 17s and 25s coding regions of P. lophurae. Analysis of quantitative genomic Southern blots reveals that there are approximately six rRNA genes per haploid genome, and that the rRNA genes may be divided into four distinct classes by restriction analysis. Examination of the flanking regions of these genes indicates that they are not organized into easily recognizable tandem repeats.

MeSH Terms
Animals Base Sequence Cloning, Molecular DNA/genetics DNA Restriction Enzymes DNA, Recombinant/metabolism DNA, Ribosomal Genes Nucleic Acid Hybridization Plasmodium/genetics RNA, Ribosomal/genetics
Chemicals
DNA, Recombinant DNA, Ribosomal RNA, Ribosomal DNA DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Unnasch T R
Wirth D F
References (21)
21 references, click to expand
  1. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.
    Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 PMID: 4894690
  2. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  3. Ribosomal RNA gene dosage as a function of tissue and age for mouse and human.
    Biochim Biophys Acta. 1976 Feb 5;418(3):358-75 PMID: 1247550
  4. The Plasmodium lophurae (avian malaria) ribosome.
    J Protozool. 1977 May;24(2):331-4 PMID: 881657
  5. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  6. Sequence arrangement of the 16S and 26S rRNA genes in the pathogenic haemoflagellate Leishmania donovani.
    Nucleic Acids Res. 1978 Feb;5(2):491-504 PMID: 634795
  7. Mapping of the Xenopus laevis 5.8S rDNA by restriction and DNA sequencing.
    Nucleic Acids Res. 1978 Apr;5(4):1121-37 PMID: 652517
  8. Preparative and analytical purification of DNA from agarose.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):615-9 PMID: 284385
  9. Sequence organization of the spacer DNA in a ribosomal gene unit of Xenopus laevis.
    Cell. 1979 May;17(1):19-31 PMID: 455459
  10. Restriction analysis of spacers in ribosomal DNA of Drosophila melanogaster.
    Nucleic Acids Res. 1979 Sep 11;7(1):205-15 PMID: 114986
  11. Localization of transcribed regions on extrachromosomal ribosomal RNA genes of Tetrahymena thermophila by R-loop mapping.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):5051-5 PMID: 291921
  12. Expression of ribosomal DNA insertions in Drosophila melanogaster.
    Cell. 1979 Dec;18(4):1185-96 PMID: 117903
  13. Biochemistry of Plasmodium (malarial parasites).
    Microbiol Rev. 1979 Dec;43(4):453-95 PMID: 94424
  14. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  15. Repeated genes in eukaryotes.
    Annu Rev Biochem. 1980;49:727-64 PMID: 6996571
  16. Length variation in the non-transcribed spacer of Calliphora erythrocephala ribosomal DNA is due to a 350 base-pair repeat.
    J Mol Biol. 1981 Feb 25;146(2):179-99 PMID: 6267296
  17. Expression of the ribosomal DNA insertions in bobbed mutants of Drosophila melanogaster.
    Mol Gen Genet. 1981;182(3):377-84 PMID: 6795426
  18. Rapid analysis of small nucleic acid samples by gel electrophoresis.
    Anal Biochem. 1981 Aug;115(2):419-23 PMID: 6272606
  19. Distribution of ribosomal gene length variants among mouse chromosomes.
    Proc Natl Acad Sci U S A. 1982 Aug;79(15):4677-80 PMID: 6956886
  20. The amino acid composition of T3 bacteriophage.
    J Biol Chem. 1953 Nov;205(1):291-5 PMID: 13117907
  21. LOCALIZATION OF DNA COMPLEMENTARY TO RIBOSOMAL RNA IN THE NUCLEOLUS ORGANIZER REGION OF DROSOPHILA MELANOGASTER.
    Proc Natl Acad Sci U S A. 1965 Apr;53:737-45 PMID: 14324529
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.23.8443
Published
1983-12-10
Pages
8443-59
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326594
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/11.23.8443 PMC Full Text View journal details
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