pFJ265, a new cloning vehicle for Streptomyces.

Plasmid ·Vol. 11 ·No. 1 ·1984-01-00 ·Pages 92-5

Jones MD, Mabe JA, Nakatsukasa WM, Fayerman JT

Abstract

A 9.3-kb plasmid, pNM100, was isolated from Streptomyces virginiae (NRRL 15156) and characterized. Streptomyces genes for thiostrepton and neomycin resistance were cloned into pNM100 to yield a small plasmid derivative, pFJ265, that is suitable for Streptomyces gene cloning. pFJ265 is a 9.2-kb nonconjugative plasmid and has a copy number of several hundred per chromosome.

MeSH Terms

Chromosome Mapping Cloning, Molecular DNA Restriction Enzymes Drug Resistance, Microbial Genes, Bacterial Plasmids Streptomyces/genetics

Chemicals

DNA Restriction Enzymes

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Jones M D

Mabe J A

Nakatsukasa W M

Fayerman J T

Article Info

Journal

Plasmid

Abbr.

Plasmid

ISSN

0147-619X

DOI

10.1016/0147-619x(84)90011-8

Published

1984-01-00

Pages

92-5

Language

English

Region

United States

NLM ID

7802221

Subset

External Links

PubMed source DOI: 10.1016/0147-619x(84)90011-8 View journal details

Analysis Services

pFJ265, a new cloning vehicle for Streptomyces.

Contact

WeChat Official Account