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PMID: 6325158 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sequence of a 1.26-kb DNA fragment containing the structural gene for E.coli initiation factor IF3: presence of an AUU initiator codon.

The EMBO journal ·Vol. 1 ·No. 3 ·1982-00-00 ·Pages 311-5

Sacerdot C, Fayat G, Dessen P, Springer M, Plumbridge JA, Grunberg-Manago M, Blanquet S

Abstract

The nucleotide sequence of a 1.26-kb pair DNA fragment containing the structural gene for Escherichia coli initiation factor IF3 has been determined. An open reading frame of 540 nucleotides is found at the position predicted by genetic studies. The amino-acid sequence deduced from the DNA sequence accounts for a molecular weight 20 530. The important feature of the coding DNA sequence is the presence of AUU as the translational initiator codon. It is 11 bases downstream of the center of a GGAGG sequence, which can strongly pair with the sequence CCUCC near the 3' terminus of 16S rRNA. The primary DNA sequence in the region of the AUU initiator codon and its role in compensating a reduced codon-anticodon interaction in initiation complex formation are discussed.

MeSH Terms

Amino Acid Sequence Base Sequence DNA Restriction Enzymes/metabolism DNA, Bacterial/analysis Genes Peptide Initiation Factors/genetics Prokaryotic Initiation Factor-3

Chemicals

DNA, Bacterial Peptide Initiation Factors Prokaryotic Initiation Factor-3 DNA Restriction Enzymes

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Sacerdot C

Fayat G

Dessen P

Springer M

Plumbridge J A

Grunberg-Manago M

Blanquet S

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25 references, click to expand

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Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

Published

1982-00-00

Pages

311-5

Language

English

Region

England

NLM ID

8208664

PMCID

PMC553041

Subset

Databases

GENBANK

M10430, V00291

External Links

PubMed source PMC Full Text View journal details

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Sequence of a 1.26-kb DNA fragment containing the structural gene for E.coli initiation factor IF3: presence of an AUU initiator codon.

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