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PMID: 6326105 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Evidence that the Rous sarcoma virus transforming gene product phosphorylates phosphatidylinositol and diacylglycerol.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 7 ·1984-04-00 ·Pages 2117-21

Sugimoto Y, Whitman M, Cantley LC, Erikson RL

Abstract

The ability of purified Rous sarcoma virus transforming gene product, pp60v-src, to phosphorylate phosphatidylinositol and diacylglycerol was investigated. Phosphatidylinositol was phosphorylated to form both mono- and diphosphorylated derivatives. 1,2-Diacylglycerol was phosphorylated to form phosphatidic acid. These activities showed the same thermolability and the same sensitivity to inhibitors as shown by the casein kinase activity of pp60v-src. In addition, when serum-starved chicken embryo fibroblasts transformed by a virus mutant temperature-sensitive for transformation were shifted from the nonpermissive to permissive temperature, an increase of 50-100% in the labeling of phosphatidylinositol 4-phosphate, phosphatidylinositol 4,5-bisphosphate, and phosphatidic acid was observed, as compared to uninfected cells.

MeSH Terms
Animals Avian Sarcoma Viruses/genetics Cattle Cell Transformation, Neoplastic Chick Embryo Diglycerides/metabolism Enzyme Activation Fibroblasts/enzymology Genes Genes, Viral Glycerides/metabolism Kinetics Mutation Oncogene Protein pp60(v-src) Phosphatidylinositols/metabolism Phosphorylation Protein Kinases/genetics,metabolism Temperature Viral Proteins/genetics,metabolism
Chemicals
Diglycerides Glycerides Phosphatidylinositols Viral Proteins Protein Kinases Oncogene Protein pp60(v-src)
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sugimoto Y
Whitman M
Cantley L C
Erikson R L
References (36)
36 references, click to expand
  1. Thin-layer chromatography of the phosphoinositides.
    J Lipid Res. 1968 Jul;9(4):532-3 PMID: 4302257
  2. Release of Ca2+ from a nonmitochondrial intracellular store in pancreatic acinar cells by inositol-1,4,5-trisphosphate.
    Nature. 1983 Nov 3-9;306(5938):67-9 PMID: 6605482
  3. The effects of reciprocal changes in temperature on the transformed state of cells infected with a rous sarcoma virus mutant.
    Virology. 1971 Nov;46(2):470-9 PMID: 4331732
  4. Preparation of homogeneous cyclic AMP-dependent protein kinase(s) and its subunits from rabbit skeletal muscle.
    Methods Enzymol. 1974;38:299-308 PMID: 4375763
  5. Changes in phosphatidylinositol metabolism correlated to growth state of normal and Rous sarcoma virus-transformed Japanese quail cells.
    Cancer Res. 1977 Sep;37(9):2979-84 PMID: 195714
  6. Identification of a transformation-specific antigen induced by an avian sarcoma virus.
    Nature. 1977 Sep 22;269(5626):346-8 PMID: 198667
  7. Protein kinase activity associated with the avian sarcoma virus src gene product.
    Proc Natl Acad Sci U S A. 1978 Apr;75(4):2021-4 PMID: 205879
  8. Evidence that the transforming gene of avian sarcoma virus encodes a protein kinase associated with a phosphoprotein.
    Cell. 1978 Oct;15(2):561-72 PMID: 214242
  9. Immunofluorescence on avian sarcoma virus-transformed cells: localization of the src gene product.
    Cell. 1979 Jan;16(1):11-24 PMID: 217542
  10. Calcium-dependent activation of a multifunctional protein kinase by membrane phospholipids.
    J Biol Chem. 1979 May 25;254(10):3692-5 PMID: 438153
  11. Production of diglyceride from phosphatidylinositol in activated human platelets.
    J Clin Invest. 1979 Apr;63(4):580-7 PMID: 220279
  12. The enzymatic preparation of [alpha-32P]ATP, [alpha-32P]GTP, [32P]cAMP, and [32P]cGMP, and their use in the assay of adenylate and guanylate cyclases and cyclic nucleotide phosphodiesterases.
    Adv Cyclic Nucleotide Res. 1979;10:135-67 PMID: 36738
  13. Phosphorylation-dephosphorylation of enzymes.
    Annu Rev Biochem. 1979;48:923-59 PMID: 38740
  14. Transformation by Rous sarcoma virus: effects of src gene expression on the synthesis and phosphorylation of cellular polypeptides.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):5212-6 PMID: 228282
  15. Localization of the ASV src gene product to the plasma membrane of transformed cells by electron microscopic immunocytochemistry.
    Cell. 1979 Sep;18(1):125-34 PMID: 228858
  16. Evidence that the src gene product of Rous sarcoma virus is membrane associated.
    Virology. 1980 Feb;101(1):25-40 PMID: 6243822
  17. Transforming gene product of Rous sarcoma virus phosphorylates tyrosine.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1311-5 PMID: 6246487
  18. Evidence that the phosphorylation of tyrosine is essential for cellular transformation by Rous sarcoma virus.
    Cell. 1980 Jul;20(3):807-16 PMID: 6251974
  19. Protein phosphorylation mediated by partially purified avian sarcoma virus transforming-gene product.
    Cold Spring Harb Symp Quant Biol. 1980;44 Pt 2,:907-17 PMID: 6253222
  20. The protein encoded by the transforming gene of avian sarcoma virus (pp60src) and a homologous protein in normal cells (pp60proto-src) are associated with the plasma membrane.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3783-7 PMID: 6253989
  21. Identification of a cellular protein substrate phosphorylated by the avian sarcoma virus-transforming gene product.
    Cell. 1980 Oct;21(3):829-36 PMID: 6254669
  22. Hormone-stimulated metabolism of inositol lipids and its relationship to hepatic receptor function.
    Biochem Soc Trans. 1981 Oct;9(5):377-9 PMID: 6269920
  23. Phosphorylation of ribosomal protein S6 in avian sarcoma virus-transformed chicken embryo fibroblasts.
    Proc Natl Acad Sci U S A. 1981 Jul;78(7):4112-5 PMID: 6270659
  24. Properties of the src kinase purified from Rous sarcoma virus-induced rat tumors.
    J Biol Chem. 1982 Jun 25;257(12):7143-50 PMID: 6282833
  25. Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters.
    J Biol Chem. 1982 Jul 10;257(13):7847-51 PMID: 7085651
  26. In vitro phosphorylation of angiotensin analogs by tyrosyl protein kinases.
    J Biol Chem. 1983 Jan 25;258(2):1022-5 PMID: 6822491
  27. Diacylglycerol kinase from pig brain. Purification and phospholipid dependencies.
    J Biol Chem. 1983 Feb 10;258(3):1767-74 PMID: 6296111
  28. Thrombin-induced phosphodiesteratic cleavage of phosphatidylinositol bisphosphate in human platelets.
    J Biol Chem. 1983 Feb 25;258(4):2076-8 PMID: 6296123
  29. Evidence that the Rous sarcoma virus transforming gene product is associated with glycerol kinase activity.
    J Biol Chem. 1983 Feb 25;258(4):2126-9 PMID: 6296132
  30. Three glycolytic enzymes are phosphorylated at tyrosine in cells transformed by Rous sarcoma virus.
    Nature. 1983 Mar 17-23;302(5905):218-23 PMID: 6188054
  31. Characterization of the Rous sarcoma virus transforming gene product.
    J Biol Chem. 1983 May 25;258(10):6344-51 PMID: 6304035
  32. Phosphorylation of phosphatidylinositol in rat liver Golgi.
    J Biol Chem. 1983 Jul 10;258(13):7968-73 PMID: 6863275
  33. Rapid accumulation of inositol trisphosphate reveals that agonists hydrolyse polyphosphoinositides instead of phosphatidylinositol.
    Biochem J. 1983 Jun 15;212(3):849-58 PMID: 6309155
  34. The effect of quercetin on the phosphorylation activity of the Rous sarcoma virus transforming gene product in vitro and in vivo.
    Eur J Biochem. 1983 Oct 3;135(3):583-9 PMID: 6311542
  35. Phosphorylation of isocitrate dehydrogenase as a demonstration of enhanced sensitivity in covalent regulation.
    Nature. 1983 Sep 22-28;305(5932):286-90 PMID: 6312317
  36. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.7.2117
Published
1984-04-00
Pages
2117-21
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC345448
Subset
IM
Grants
NCI NIH HHS · CA34943 · United States
NIGMS NIH HHS · GM28538 · United States
External Links
PubMed source DOI: 10.1073/pnas.81.7.2117 PMC Full Text View journal details
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