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PMID: 6327068 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The gene family encoding the mouse ribosomal protein L32 contains a uniquely expressed intron-containing gene and an unmutated processed gene.

Cell ·Vol. 37 ·No. 2 ·1984-06-00 ·Pages 457-68

Dudov KP, Perry RP

Abstract

The family of approximately 16 genes encoding the mouse ribosomal protein L32 has been characterized by an analysis of a representative set of genomic clones. Surprisingly, this family contains only a single expressed intron-containing gene. This gene, termed rpL32 , has been completely sequenced and found to possess certain novel features including the presence in two of its introns of a sequence with high homology to the 5' end of U1 snRNA and a 5' terminal region exceptionally rich in pyrimidines. Most of the other members of the L32 family appear to be processed genes, some of which are identical or very similar to the rpL32 gene, except for the lack of introns. One unmutated gene was found to be integrated 28 nucleotides downstream of a canonical TATA box. However, despite this feature, the gene does not seem to be expressed, as judged by its extent of methylation compared to the expressed rpL32 gene.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Line Cell Nucleus/metabolism DNA/analysis DNA Restriction Enzymes Genes Mice Nucleic Acid Denaturation Nucleic Acid Hybridization Plasmacytoma Ribosomal Proteins/genetics Transcription, Genetic
Chemicals
Ribosomal Proteins ribosomal protein L32 DNA DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Dudov K P
Perry R P
Article Info
Journal
Cell
Abbr.
Cell
ISSN
0092-8674
Published
1984-06-00
Pages
457-68
Language
English
Region
United States
NLM ID
0413066
Subset
IM
Databases
GENBANK
K02060, K02061
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