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PMID: 6327258 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

SV40 virions and viral RNA metabolism are associated with cellular substructures.

The EMBO journal ·Vol. 1 ·No. 10 ·1982-00-00 ·Pages 1225-31

Ben-Ze'ev A, Abulafia R, Aloni Y

Abstract

Nuclear matrices were prepared by DNase and high salt extraction of SV40-infected epithelial monkey cells. The matrices retain the majority of SV40 virions. This conclusion is based on electron microscopic observations of the occurrence of encapsidated viral DNA that is resistant to DNase digestion and on the analysis of viral proteins by gel electrophoresis. Pulse labeled SV40 RNA is also associated with the nuclear matrix (less than 15% of the viral RNA is removed by DNase and high salt). Pulse-chase experiments revealed that processing of SV40 RNA takes place on the nuclear matrix and the processed molecules are directly transported to the cytoplasm where they are associated with the cytoskeleton. These results suggest a central role for the nuclear and cytoplasmic substructures in virus assembly and in the biogenesis of viral RNA.

MeSH Terms
Animals Cell Line Cell Nucleus/metabolism,ultrastructure Cell Transformation, Viral Chlorocebus aethiops Epithelium Kinetics Microscopy, Electron RNA, Viral/metabolism Simian virus 40/metabolism,ultrastructure Virion/metabolism,ultrastructure
Chemicals
RNA, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ben-Ze'ev A
Abulafia R
Aloni Y
References (31)
31 references, click to expand
  1. Biogenesis and characterization of SV40 and polyoma RNAs in productively infected cells.
    Cold Spring Harb Symp Quant Biol. 1975;39 Pt 1:165-78 PMID: 169056
  2. Temperature-sensitive B mutants of SV40 disassemble intracellular encapsidation particles at elevated temperature.
    Virology. 1982 Aug;121(1):95-106 PMID: 6287723
  3. Reversibly contractile nuclear matrix. Its isolation, structure, and composition.
    J Cell Biol. 1977 May;73(2):271-8 PMID: 404299
  4. Removal of RNase activity from DNase by affinity chromatography on agarose coupled aminophenylphosphoryl-uridine-2' (3')-phosphate.
    Nucleic Acids Res. 1977 Jan;4(1):241-6 PMID: 866176
  5. The metabolism of poly (A)+ and poly(A)-hnRNA in cultured Drosophila cells studied with a rapid uridine pulse-chase.
    Cell. 1977 May;11(1):105-13 PMID: 872216
  6. Role of cell shape in growth control.
    Nature. 1978 Jun 1;273(5661):345-9 PMID: 661946
  7. The control of mRNA production, translation and turnover in suspended and reattached anchorage-dependent fibroblasts.
    Cell. 1978 Aug;14(4):931-9 PMID: 688399
  8. Determination of cellular shape by the extracellular matrix and its correlation with the control of cellular growth.
    Cancer Res. 1978 Nov;38(11 Pt 2):4155-71 PMID: 359133
  9. Heterogeneous nuclear RNA-protein fibers in chromatin-depleted nuclei.
    J Cell Biol. 1978 Sep;78(3):663-74 PMID: 701354
  10. Selective degradation of newly synthesized nonmessenger simian virus 40 transcripts.
    J Virol. 1978 Nov;28(2):590-9 PMID: 214580
  11. The initiation of transcription of SV40 DNA at late time after infection.
    Virology. 1979 Jan 30;92(2):310-23 PMID: 218346
  12. The cytoskeletal framework and poliovirus metabolism.
    Cell. 1979 Feb;16(2):289 301 PMID: 222454
  13. The outer boundary of the cytoskeleton: a lamina derived from plasma membrane proteins.
    Cell. 1979 Aug;17(4):859-65 PMID: 573666
  14. Isolation and characterization of the nuclear matrix in Friend erythroleukemia cells: chromatin and hnRNA interactions with the nuclear matrix.
    Cell. 1979 Dec;18(4):1079-90 PMID: 293220
  15. The relationship of the nuclear matrix to cellular structure and function.
    Adv Enzyme Regul. 1978;17:213-48 PMID: 393089
  16. A fixed site of DNA replication in eucaryotic cells.
    Cell. 1980 Feb;19(2):527-36 PMID: 7357619
  17. Isolation and characterization of various forms of simian virus 40 DNA-protein complexes.
    Virology. 1980 Apr 15;102(1):107-18 PMID: 6245499
  18. Protein synthesis requires cell-surface contact while nuclear events respond to cell shape in anchorage-dependent fibroblasts.
    Cell. 1980 Sep;21(2):365-72 PMID: 6157481
  19. Association of polyoma T antigen and DNA with the nuclear matrix from lytically infected 3T6 cells.
    Cell. 1980 Nov;22(1 Pt 1):37-46 PMID: 6253084
  20. Nuclear non-chromatin proteinaceous structures: their role in the organization and function of the interphase nucleus.
    J Cell Sci. 1980 Aug;44:395-435 PMID: 7002943
  21. Fluorescence microscopical analysis of the life cycle of vaccinia virus in chick embryo fibroblasts. Virus-cytoskeleton interactions.
    Exp Cell Res. 1981 Mar;132(1):81-7 PMID: 7193591
  22. Messenger RNA is translated when associated with the cytoskeletal framework in normal and VSV-infected HeLa cells.
    Cell. 1981 Jan;23(1):113-20 PMID: 6260369
  23. hnRNA and its attachment to a nuclear protein matrix.
    J Cell Biol. 1981 Mar;88(3):554-63 PMID: 7217204
  24. The metabolism of SV40 RNA is associated with the cytoskeletal framework.
    Virology. 1981 Jun;111(2):475-87 PMID: 6264669
  25. RNA is synthesized at the nuclear cage.
    Nature. 1981 Aug 6;292(5823):552-5 PMID: 6166873
  26. On the association of mRNA with the cytoskeleton in uninfected and adenovirus-infected human KB cells.
    Exp Cell Res. 1981 Sep;135(1):79-91 PMID: 7286081
  27. Influenza virus RNA is synthesized at fixed sites in the nucleus.
    Nature. 1982 Mar 25;296(5855):366-8 PMID: 7063035
  28. RNA metabolism in the HeLa cell nucleus.
    J Mol Biol. 1966 May;17(1):117-30 PMID: 5961144
  29. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  30. Patterns of organization of actin and myosin in normal and transformed cultured cells.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):994-8 PMID: 165499
  31. Isolation of a nuclear ribonucleoprotein network that contains heterogeneous RNA and is bound to the nuclear envelope.
    Biochemistry. 1975 Aug 26;14(17):3808-16 PMID: 1174505
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1982-00-00
Pages
1225-31
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553193
Subset
IM
Grants
NCI NIH HHS · CA-14995 · United States
External Links
PubMed source PMC Full Text View journal details
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