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PMID: 6327275 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

DNA sequences required for transcription in vivo of the human corticotropin-beta-lipotropin precursor gene.

The EMBO journal ·Vol. 1 ·No. 12 ·1982-00-00 ·Pages 1533-8

Mishina M, Kurosaki T, Yamamoto T, Notake M, Masu M, Numa S

Abstract

The cloned human corticotropin-beta-lipotropin precursor gene, when joined with an SV40 vector and introduced into COS monkey cells, is transcribed from its own promoter. The DNA sequences required for promoter function have been identified by using 5' deletion mutants of the fusion gene AT which contains the 5'-flanking sequence and capping site of the human corticotropin-beta-lipotropin precursor gene and the structural sequence of the herpes simplex virus thymidine kinase gene. The deletion of the sequence located between 53 and 59 bp upstream of the capping site enhances the transcription approximately 3-fold, while the deletion of the TATA box region abolishes the transcription.

MeSH Terms
Adrenocorticotropic Hormone/genetics Animals Base Sequence Chlorocebus aethiops DNA/genetics DNA Restriction Enzymes DNA, Recombinant/metabolism Genes Humans Kidney Operon Pituitary Hormones, Anterior/genetics Plasmids Pro-Opiomelanocortin Protein Precursors/genetics Transcription, Genetic Transfection beta-Lipotropin/genetics
Chemicals
DNA, Recombinant Pituitary Hormones, Anterior Protein Precursors Pro-Opiomelanocortin Adrenocorticotropic Hormone DNA beta-Lipotropin DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Mishina M
Kurosaki T
Yamamoto T
Notake M
Masu M
Numa S
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41 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1982-00-00
Pages
1533-8
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553247
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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