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PMID: 6327616 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

D-ribose metabolism in Escherichia coli K-12: genetics, regulation, and transport.

Journal of bacteriology ·Vol. 158 ·No. 2 ·1984-05-00 ·Pages 665-73

Lopilato JE, Garwin JL, Emr SD, Silhavy TJ, Beckwith JR

Abstract

We have isolated mutants defective in high-affinity D-ribose transport. The mutations map in rbsT or rbsB , the structural gene for ribose binding protein. rbsT consists of at least one gene coding for a protein required for high-affinity transport. The high-affinity transport-defective mutants were able to utilize D-ribose, indicating that at least a second, low-affinity transport system for D-ribose is present in Escherichia coli K-12. rbsT and rbsB are located at min 84 on the E. coli genetic map and, together with rbsK , the gene coding for ribokinase , constitute an rbs operon. The order of genes is rbsP /O rbsT rbsB rbsK . The rbs operon is subject to negative control by the product of the rbsR gene. rbsR is located distal to the rbs operon and appears to form a separate transcriptional unit.

MeSH Terms
Bacterial Proteins/genetics Biological Transport Carrier Proteins/genetics Chromosome Mapping Chromosomes, Bacterial Escherichia coli/genetics,metabolism Escherichia coli Proteins Genes Genes, Bacterial Genes, Regulator Mutation Operon Periplasmic Binding Proteins Phosphotransferases/genetics Phosphotransferases (Alcohol Group Acceptor) Ribose/metabolism
Chemicals
Bacterial Proteins Carrier Proteins Escherichia coli Proteins Periplasmic Binding Proteins RbsB protein, E coli Ribose Phosphotransferases Phosphotransferases (Alcohol Group Acceptor) ribokinase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lopilato J E
Garwin J L
Emr S D
Silhavy T J
Beckwith J R
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.158.2.665-673.1984
Published
1984-05-00
Pages
665-73
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215481
Subset
IM
Grants
NCI NIH HHS · N01-CO-23909 · United States
External Links
PubMed source DOI: 10.1128/jb.158.2.665-673.1984 PMC Full Text View journal details
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