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PMID: 6328015 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Oncogenicity by adenovirus is not determined by the transforming region only.

Journal of virology ·Vol. 50 ·No. 3 ·1984-06-00 ·Pages 847-53

Bernards R, de Leeuw MG, Vaessen MJ, Houweling A, van der Eb AJ

Abstract

We have constructed a nondefective recombinant virus between the nononcogenic adenovirus 5 (Ad5) and the highly oncogenic Ad12. The recombinant genome consists essentially of Ad5 sequences, with the exception of the transforming early region 1 (E1) which is derived from Ad12. HeLa cells infected with the recombinant virus were shown to contain the Ad12-specific E1 proteins of 41 kilodaltons (E1a) and 19 and 54 kilodaltons (both encoded by E1b). The recombinant virus replicated efficiently in human embryonic kidney cells and HeLa cells, showing that the transforming regions of Ad5 and Ad12 had similar functions in productive infection. After the recombinant virus was injected into newborn hamsters, no tumors were produced during an observation period of 200 days. Thus, despite the fact that all products required for oncogenic transformation in vitro were derived from the highly oncogenic Ad12, the recombinant virus did not produce tumors in vivo. These data show that tumor induction by adenovirus virions is not determined only by the gene products of the transforming region.

MeSH Terms
Adenoviruses, Human/genetics Animals Base Sequence Cell Line Cell Transformation, Neoplastic Cloning, Molecular Cricetinae DNA Restriction Enzymes DNA, Recombinant/metabolism DNA, Viral/genetics HeLa Cells/physiology Humans Kidney/embryology Phenotype Plasmids
Chemicals
DNA, Recombinant DNA, Viral DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Bernards R
de Leeuw M G
Vaessen M J
Houweling A
van der Eb A J
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39 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.50.3.847-853.1984
Published
1984-06-00
Pages
847-53
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255745
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.50.3.847-853.1984 PMC Full Text View journal details
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