Home LiteratureArticle Details
PMID: 6328279 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Drosophila melanogaster has only one myosin alkali light-chain gene which encodes a protein with considerable amino acid sequence homology to chicken myosin alkali light chains.

Molecular and cellular biology ·Vol. 4 ·No. 5 ·1984-05-00 ·Pages 956-65

Falkenthal S, Parker VP, Mattox WW, Davidson N

Abstract

A chimeric lambda DNA molecule containing the myosin alkali light-chain gene of Drosophila melanogaster was isolated. The encoded amino acid sequence was determined from the nucleic acid sequence of a cDNA homologous to the genomic clone. The identity of the encoded protein was established by two criteria: (i) sequence homology with the chicken alkali light-chain proteins and (ii) comparison of the two-dimensional gel electrophoretic pattern of the peptides synthesized by in vitro translation of hybrid-selected RNA to that of myosin alkali light-chain peptides extracted from Drosophila myofibrils. There is only one myosin alkali light-chain in D. melanogaster; its chromosomal location is region 98B . This gene is abundantly expressed during the development of larval as well as adult muscles. The Drosophila protein appears to contain one putative divalent cation-binding domain (an EF hand) as compared with the three EF hands present in chicken alkali light chains.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Chickens Cloning, Molecular DNA/metabolism DNA Restriction Enzymes Drosophila melanogaster/genetics Genes Hydrogen-Ion Concentration Muscles/metabolism Myosins/genetics Species Specificity
Chemicals
DNA DNA Restriction Enzymes Myosins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Falkenthal S
Parker V P
Mattox W W
Davidson N
References (37)
37 references, click to expand
  1. Two-dimensional gel patterns of protein synthesis before and after fertilization of sea urchin eggs.
    Dev Biol. 1976 Sep;52(2):310-7 PMID: 12194440
  2. THIRD COMPONENT PARTICIPATING IN THE SUPERPRECIPITATION OF 'NATURAL ACTOMYOSIN'.
    Nature. 1963 Dec 7;200:1010 PMID: 14097720
  3. Light chains from fast and slow muscle myosins.
    Nature. 1971 Nov 12;234(5324):81-5 PMID: 4942892
  4. Substructure of the myosin molecule. II. The light chains of myosin.
    J Mol Biol. 1971 Nov 14;61(3):701-25 PMID: 4257244
  5. The amino acid sequence of rabbit skeletal muscle troponin C: gene replication and homology with calcium-binding proteins from carp and hake muscle.
    FEBS Lett. 1973 Nov 1;36(3):268-72 PMID: 4797035
  6. High resolution two-dimensional electrophoresis of proteins.
    J Biol Chem. 1975 May 25;250(10):4007-21 PMID: 236308
  7. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
    Eur J Biochem. 1975 Aug 15;56(2):335-41 PMID: 1175627
  8. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  9. Rat insulin genes: construction of plasmids containing the coding sequences.
    Science. 1977 Jun 17;196(4296):1313-9 PMID: 325648
  10. The isolation of structural genes from libraries of eucaryotic DNA.
    Cell. 1978 Oct;15(2):687-701 PMID: 719759
  11. Identification of a novel form of myosin light chain present in embryonic muscle tissue and cultured muscle cells.
    J Mol Biol. 1978 Dec 15;126(3):415-31 PMID: 745235
  12. Rates of synthesis of major classes of RNA in Drosophila embryos.
    Dev Biol. 1979 May;70(1):217-31 PMID: 110635
  13. Purification and mapping of specific mRNAs by hybridization-selection and cell-free translation.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):4927-31 PMID: 291909
  14. The complete amino acid sequence of the Ca2+-dependent modulator protein (calmodulin) of bovine brain.
    J Biol Chem. 1980 Feb 10;255(3):962-75 PMID: 7356670
  15. The actin genes of Drosophila: a dispersed multigene family.
    Cell. 1980 Feb;19(2):365-78 PMID: 6244106
  16. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  17. Structure and evolution of calcium-modulated proteins.
    CRC Crit Rev Biochem. 1980;8(2):119-74 PMID: 6105043
  18. Calmodulin.
    Annu Rev Biochem. 1980;49:489-515 PMID: 6250447
  19. The phosphorylated L2 light chain of skeletal myosin is a modifier of the actomyosin ATPase.
    J Biol Chem. 1980 Sep 25;255(18):8836-41 PMID: 6447700
  20. The gross anatomy of a tRNA gene cluster at region 42A of the D. melanogaster chromosome.
    Cell. 1980 Nov;22(1 Pt 1):137-48 PMID: 6253076
  21. Sequence organization of feline leukemia virus DNA in infected cells.
    Nucleic Acids Res. 1980 Aug 11;8(15):3287-305 PMID: 6255412
  22. The primary structure of L-1 light chain of chicken fast skeletal muscle myosin and its genetic implication.
    FEBS Lett. 1981 Apr 6;126(1):111-3 PMID: 7238855
  23. Sequence arrangement and biological activity of cloned feline leukemia virus proviruses from a virus-productive human cell line.
    J Virol. 1981 May;38(2):688-703 PMID: 6264136
  24. Hydrolysis of ATP and reversible binding to F-actin by myosin heavy chains free of all light chains.
    Nature. 1981 Aug 6;292(5823):560-2 PMID: 6454850
  25. The cuticle genes of drosophila: a developmentally regulated gene cluster.
    Cell. 1981 Jul;25(1):165-77 PMID: 6168386
  26. An improved procedure for utilizing terminal transferase to add homopolymers to the 3' termini of DNA.
    Nucleic Acids Res. 1981 Aug 25;9(16):4173-88 PMID: 6272197
  27. Amino acid sequences of the cardiac L-2A, L-2B and gizzard 17 000-Mr light chains of chicken muscle myosin.
    FEBS Lett. 1981 Dec 7;135(2):232-6 PMID: 7319048
  28. Molecular organization of a Drosophila puff site that responds to ecdysone.
    Cell. 1982 Jan;28(1):165-76 PMID: 6279311
  29. Identification of Drosophila indirect flight muscle myofibrillar proteins by means of two-dimensional electrophoresis.
    J Biochem. 1982 Feb;91(2):643-50 PMID: 6802813
  30. High-efficiency cloning of full-length cDNA.
    Mol Cell Biol. 1982 Feb;2(2):161-70 PMID: 6287227
  31. Molecular cloning and nucleotide sequences of the complementary DNAs to chicken skeletal muscle myosin two alkali light chain mRNAs.
    Nucleic Acids Res. 1982 Oct 11;10(19):6099-110 PMID: 6128725
  32. Cuticle protein genes of Drosophila: structure, organization and evolution of four clustered genes.
    Cell. 1982 Jul;29(3):1027-40 PMID: 6817923
  33. Drosophila has one myosin heavy-chain gene with three developmentally regulated transcripts.
    Cell. 1983 Jan;32(1):23-34 PMID: 6402306
  34. Organization and expression of Drosophila tropomyosin genes.
    J Mol Biol. 1982 Dec 5;162(2):231-50 PMID: 6186817
  35. Drosophila muscle myosin heavy chain encoded by a single gene in a cluster of muscle mutations.
    Nature. 1983 Mar 31-Apr 6;302(5907):393-7 PMID: 6403869
  36. Transcripts of the six Drosophila actin genes accumulate in a stage- and tissue-specific manner.
    Cell. 1983 May;33(1):115-23 PMID: 6432334
  37. Light chains of myosins from white, red, and cardiac muscles.
    Proc Natl Acad Sci U S A. 1971 May;68(5):946-50 PMID: 4252540
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.4.5.956-965.1984
Published
1984-05-00
Pages
956-65
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368847
Subset
IM
Grants
NIGMS NIH HHS · 1 F32 GM08895-01 · United States
Databases
GENBANK
K01567
External Links
PubMed source DOI: 10.1128/mcb.4.5.956-965.1984 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2