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PMID: 6328283 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Toxicity of 2-deoxygalactose to Saccharomyces cerevisiae cells constitutively synthesizing galactose-metabolizing enzymes.

Molecular and cellular biology ·Vol. 4 ·No. 5 ·1984-05-00 ·Pages 994-6

Platt T

Abstract

Analysis of 400 independent spontaneous mutations conferring 2-deoxygalactose resistance upon cells constitutive for the galactose pathway suggests that toxicity is due to 2-deoxygalactose-1-phosphate. Selection for and against growth on galactose in the same strain is now possible; application to systems with transcriptional or translational gene fusions to galactokinase are discussed.

MeSH Terms
Carbohydrate Epimerases/genetics Drug Resistance Galactokinase/genetics Galactose/analogs & derivatives,metabolism,toxicity Genes/drug effects Genes, Fungal/drug effects Genetic Complementation Test Mutation Nucleotidyltransferases/genetics Saccharomyces cerevisiae/drug effects,enzymology UDPglucose 4-Epimerase/genetics UTP-Hexose-1-Phosphate Uridylyltransferase/genetics
Chemicals
2-deoxy-lyxo-hexose Galactokinase Nucleotidyltransferases UTP-Hexose-1-Phosphate Uridylyltransferase Carbohydrate Epimerases UDPglucose 4-Epimerase Galactose
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Platt T
References (27)
27 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.4.5.994-996.1984
Published
1984-05-00
Pages
994-6
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368858
Subset
IM
Grants
NIAID NIH HHS · AI18738 · United States
NCRR NIH HHS · BRSG RR05358 · United States
External Links
PubMed source DOI: 10.1128/mcb.4.5.994-996.1984 PMC Full Text View journal details
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