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PMID: 6328409 Published · ppublish English Journal Article

Initiation and termination signals for transcription in bacteriophage M13.

Nucleic acids research ·Vol. 12 ·No. 10 ·1984-05-25 ·Pages 4071-81

Smits MA, Jansen J, Konings RN, Schoenmakers JG

Abstract

Transcription of the infrequently expressed phage M13 genome domain, comprising genes III, VI, I and IV, has been studied in detail by hybridization and S1-nuclease mapping studies. The contiguous genes III and VI are transcribed via an 1800 nucleotide-long RNA molecule that is initiated at a promoter which overlaps with the Rho-independent termination signal between genes III and VIII. Its synthesis is terminated at a Rho-dependent terminator in the proximal part of gene I. Transcription of gene I is not mediated by an independent promoter but most probably by read-through of RNA-polymerase through this terminator. Transcription of gene IV is accomplished by synthesis of four distinct RNAs of about 1500 to 1680 nucleotides long which are initiated at a promoter located immediately in front of gene IV. Termination of these transcripts is generated at least four different sites located in tandem within the intergenic region between genes IV and II.

MeSH Terms
Base Sequence Coliphages/genetics DNA Restriction Enzymes Endonucleases Escherichia coli/genetics Genes, Bacterial Genes, Viral Nucleic Acid Hybridization Rho Factor/genetics Single-Strand Specific DNA and RNA Endonucleases Transcription Factors/genetics Transcription, Genetic
Chemicals
Rho Factor Transcription Factors Endonucleases DNA Restriction Enzymes Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Smits M A
Jansen J
Konings R N
Schoenmakers J G
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28 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.10.4071
Published
1984-05-25
Pages
4071-81
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318817
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/12.10.4071 PMC Full Text View journal details
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