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PMID: 6328415 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Functional analysis of the steroid hormone control region of mouse mammary tumor virus.

Nucleic acids research ·Vol. 12 ·No. 10 ·1984-05-25 ·Pages 4191-206

Lee F, Hall CV, Ringold GM, Dobson DE, Luh J, Jacob PE

Abstract

Gene fusions between the mouse mammary tumor virus long terminal repeat and the E. coli lacZ gene have been shown to exhibit hormone dependent expression of beta-galactosidase activity. These constructions were used in transient expression experiments to assess the effects of specific modifications introduced into the region upstream of the transcription initiation site. 5' deletions demonstrate that sequences sufficient for wild-type promoter function are contained downstream of residue -64 relative to the initiation site. Other deletions define a region of approximately 80 base pairs between -220 and -140 which contains sequences essential for hormonal control. Between this control region and the promoter lie sequences dispensable for both functions.

MeSH Terms
Animals Base Sequence Chromosome Deletion DNA Restriction Enzymes Dexamethasone/pharmacology Escherichia coli/enzymology,genetics Galactosidases/genetics Genes, Bacterial/drug effects Genes, Viral/drug effects L Cells/drug effects Mammary Tumor Virus, Mouse/drug effects,genetics Mice Mutation Plasmids Repetitive Sequences, Nucleic Acid beta-Galactosidase/genetics
Chemicals
Dexamethasone DNA Restriction Enzymes Galactosidases beta-Galactosidase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Lee F
Hall C V
Ringold G M
Dobson D E
Luh J
Jacob P E
References (29)
29 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.10.4191
Published
1984-05-25
Pages
4191-206
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318826
Subset
IM
Grants
NIGMS NIH HHS · GM25821 · United States
External Links
PubMed source DOI: 10.1093/nar/12.10.4191 PMC Full Text View journal details
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