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PMID: 6328443 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequences of bovine alpha S1- and kappa-casein cDNAs.

Nucleic acids research ·Vol. 12 ·No. 9 ·1984-05-11 ·Pages 3895-907

Stewart AF, Willis IM, Mackinlay AG

Abstract

The nucleotide sequences corresponding to bovine alpha S1- and kappa-casein mRNAs are presented. An unusual alpha S1-casein cDNA has been characterised whose 5' end commences upstream from its putative TATA box. The alpha S1-casein mRNA is compared to rat alpha-casein mRNA and two components of divergence are identified. Firstly, the two sequences have diverged at a high point mutation rate and the rate of amino acid replacement by this mechanism is at least as great as the rate of divergence of any other part of the mRNAs. Secondly, the protein coding sequence has been subjected to several insertion/deletion events, one of which may be an example of exon shuffling . The kappa-casein mRNA sequence verifies the proposition that it has arisen from a different ancestral gene to the other caseins.

MeSH Terms
Animals Base Sequence Caseins/genetics Cattle Cloning, Molecular DNA/isolation & purification DNA Restriction Enzymes Nucleic Acid Hybridization Plasmids RNA, Messenger/genetics
Chemicals
Caseins RNA, Messenger DNA DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Stewart A F
Willis I M
Mackinlay A G
References (38)
38 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.9.3895
Published
1984-05-11
Pages
3895-907
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318797
Subset
IM
Databases
GENBANK
X00564, X00565
External Links
PubMed source DOI: 10.1093/nar/12.9.3895 PMC Full Text View journal details
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