Home LiteratureArticle Details
PMID: 6328487 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Synthesis and characterization of cDNA encoding a cartilage-specific short collagen.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 10 ·1984-05-00 ·Pages 3014-8

Ninomiya Y, Olsen BR

Abstract

Hyaline cartilage contains a unique set of collagenous proteins. Type II collagen is the most abundant, constituting about 85% of the total cartilage collagen. In addition, several minor collagenous components have been described. To study the structure and developmental regulation of chondrocyte-specific collagens, we have constructed a cDNA library from embryonic chicken sternal cartilage mRNA. We report here on the isolation and characterization of a 3200 base-pair-long cDNA that codes for a collagenous polypeptide of unusual structure in that the total length of the molecule is only about half of pro alpha 1(II) collagen chains. The mRNA for this polypeptide is considerably smaller than mRNA encoding the pro alpha chains of interstitial collagens. In addition, the peptide encoded by the cDNA appears to contain at least three domains with triple-helical potential separated by short, noncollagenous peptides. Between the three collagenous domains are several cysteinyl residues.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cartilage/metabolism Chick Embryo Cloning, Molecular Collagen/genetics DNA/metabolism DNA Restriction Enzymes DNA, Recombinant/analysis Extraembryonic Membranes/metabolism Molecular Weight
Chemicals
DNA, Recombinant Collagen DNA DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ninomiya Y
Olsen B R
References (30)
30 references, click to expand
  1. Isolation of a unique collagenous fraction from limited pepsin digests of human placental tissue. Characterization of one of the constituent polypeptide chains.
    J Biol Chem. 1980 Jan 10;255(1):290-5 PMID: 6985609
  2. Isolation and translation of elastin mRNA from chick aorta.
    Biochem Biophys Res Commun. 1979 Feb 14;86(3):478-84 PMID: 426796
  3. Structurally distinct collagen types.
    Annu Rev Biochem. 1980;49:957-1003 PMID: 6157354
  4. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  5. Identification of a new disulphide bonded collagen from cartilage.
    FEBS Lett. 1980 Nov 17;121(1):51-4 PMID: 7461120
  6. A cysteine-rich collagenous protein from bovine placenta. Isolation of its constituent polypeptide chains and some properties of the non-denatured protein.
    Eur J Biochem. 1981;114(1):17-25 PMID: 6783402
  7. Characterization of a unique collagenous fraction from limited pepsin digests of human placental tissue: molecular organization of the native aggregate.
    Biochemistry. 1981 Mar 17;20(6):1635-40 PMID: 6261803
  8. Isolation and characterisation of an unusual collagen from hyaline cartilage and intervertebral disc.
    FEBS Lett. 1981 Jan 26;123(2):195-9 PMID: 7227511
  9. A rapid boiling method for the preparation of bacterial plasmids.
    Anal Biochem. 1981 Jun;114(1):193-7 PMID: 6269464
  10. Minor collagens of chicken hyaline cartilage.
    Biochemistry. 1981 Sep 15;20(19):5443-8 PMID: 7028104
  11. Identification of two further collagenous fractions from articular cartilage.
    Biosci Rep. 1981 Jul;1(7):561-70 PMID: 7295904
  12. Characterization of a highly soluble collagenous molecule isolated from chicken hyaline cartilage.
    Biochemistry. 1982 Mar 2;21(5):826-30 PMID: 6803829
  13. Characterisation of another short-chain disulphide-bonded collagen from cartilage, vitreous and intervertebral disc.
    FEBS Lett. 1982 Mar 22;139(2):300-4 PMID: 7075781
  14. Collagen: an overview.
    Methods Enzymol. 1982;82 Pt A:3-32 PMID: 7078440
  15. Isolation and characterization of new collagens from chick cartilage.
    Eur J Biochem. 1982 May;124(1):57-62 PMID: 7084229
  16. High-efficiency cloning of full-length cDNA.
    Mol Cell Biol. 1982 Feb;2(2):161-70 PMID: 6287227
  17. A unique low molecular weight collagen secreted by cultured chick embryo chondrocytes.
    J Biol Chem. 1982 Oct 25;257(20):12444-50 PMID: 7118948
  18. Effects of matrix macromolecules on chondrocyte gene expression: synthesis of a low molecular weight collagen species by cells cultured within collagen gels.
    J Cell Biol. 1982 Jun;93(3):767-74 PMID: 6811598
  19. Further characterization of the three polypeptide chains of bovine and human short-chain collagen (intima collagen).
    Eur J Biochem. 1983 Jun 1;133(1):39-46 PMID: 6852033
  20. Isolation and partial characterization of a new human collagen with an extended triple-helical structural domain.
    Proc Natl Acad Sci U S A. 1983 Jun;80(11):3168-72 PMID: 6574478
  21. Structural diversity and domain composition of a unique collagenous fragment (intima collagen) obtained from human placenta.
    Biochem J. 1983 May 1;211(2):295-302 PMID: 6870834
  22. Electron-microscopical approach to a structural model of intima collagen.
    Biochem J. 1983 May 1;211(2):303-11 PMID: 6307276
  23. Identification and partial characterization of three low-molecular-weight collagenous polypeptides synthesized by chondrocytes cultured within collagen gels in the absence and in the presence of fibronectin.
    Biochem J. 1983 May 1;211(2):417-26 PMID: 6870839
  24. A short chain (pro)collagen from aged endochondral chondrocytes. Biochemical characterization.
    J Biol Chem. 1983 Aug 10;258(15):9504-9 PMID: 6874699
  25. Analysis of the 3' end of the human pro-alpha 2(I) collagen gene. Utilization of multiple polyadenylation sites in cultured fibroblasts.
    J Biol Chem. 1983 Aug 25;258(16):10128-35 PMID: 6309769
  26. p-HMW-collagen, a minor collagen obtained from chick embryo cartilage without proteolytic treatment of the tissue.
    Eur J Biochem. 1983 Nov 2;136(2):333-9 PMID: 6414813
  27. Isolation and characterization of a precursor form of M collagen from embryonic chicken cartilage.
    Eur J Biochem. 1984 Feb 1;138(3):629-33 PMID: 6692838
  28. The molecular structure of collagen.
    J Mol Biol. 1961 Oct;3:483-506 PMID: 14491907
  29. Levels of translatable mRNAs for cell surface protein, collagen precursors, and two membrane proteins are altered in Rous sarcoma virus-transformed chick embryo fibroblasts.
    Proc Natl Acad Sci U S A. 1977 Aug;74(8):3399-403 PMID: 198783
  30. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.10.3014
Published
1984-05-00
Pages
3014-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC345211
Subset
IM
Grants
NIADDK NIH HHS · AM 21471 · United States
Databases
GENBANK
K01702
External Links
PubMed source DOI: 10.1073/pnas.81.10.3014 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2