Home LiteratureArticle Details
PMID: 6329723 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The nucleotide sequence of the dnaA gene promoter and of the adjacent rpmH gene, coding for the ribosomal protein L34, of Escherichia coli.

The EMBO journal ·Vol. 1 ·No. 9 ·1982-00-00 ·Pages 1043-8

Hansen FG, Hansen EB, Atlung T

Abstract

The nucleotide sequence was determined of a 945-bp EcoRI fragment from the Escherichia coli K-12 chromosome at 82 min containing the promoter region of the dnaA gene. This nucleotide sequence contained a coding sequence identical to the amino acid sequence of the ribosomal protein L34 , designated rpmH . The rimA mutation, which affects the maturation of 50S ribosomal particles, may be an allele of the rpmH gene since it maps close to, or within, the L34 coding sequence. The rpmH gene and the dnaA gene are transcribed in the clockwise and counter-clockwise direction, respectively. Nuclease S1 mapping of transcripts indicated the existence of two major promoters for the L34 gene and two promoters for the dnaA gene within the 945-bp EcoRI fragment.

MeSH Terms
Alleles Bacteriophage lambda/genetics Base Sequence DNA Restriction Enzymes Endonucleases Genes Genes, Bacterial Genetic Complementation Test Mutation Operon Plasmids Ribosomal Proteins/genetics Single-Strand Specific DNA and RNA Endonucleases Transcription, Genetic
Chemicals
Ribosomal Proteins ribosomal protein L34 Endonucleases DNA Restriction Enzymes Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hansen F G
Hansen E B
Atlung T
References (39)
39 references, click to expand
  1. Regulatory sequences involved in the promotion and termination of RNA transcription.
    Annu Rev Genet. 1979;13:319-53 PMID: 94251
  2. Characterization of the dnaA, gyrB and other genes in the dnaA region of the Escherichia coli chromosome on specialized transducing phages lambda tna.
    Mol Gen Genet. 1979 Sep;175(2):135-44 PMID: 160000
  3. Characterization of dnaA gene carried by lambda transducing phage.
    Mol Gen Genet. 1980;180(2):235-47 PMID: 6450869
  4. Cloning and physical mapping of the dnaA region of the Escherichia coli chromosome.
    J Bacteriol. 1979 Dec;140(3):817-24 PMID: 160412
  5. Analysis of gene control signals by DNA fusion and cloning in Escherichia coli.
    J Mol Biol. 1980 Apr;138(2):179-207 PMID: 6997493
  6. Sea urchin histone mRNA termini are located in gene regions downstream from putative regulatory sequences.
    Nature. 1980 May 15;285(5761):147-51 PMID: 7374764
  7. Linkage map of Escherichia coli K-12, edition 6.
    Microbiol Rev. 1980 Mar;44(1):1-56 PMID: 6997720
  8. In vitro synthesis of bacteriophage lysozyme.
    Nature. 1967 Aug 5;215(5101):588-91 PMID: 6050209
  9. A versatile primer for DNA sequencing in the M13mp2 cloning system.
    Gene. 1980 Jun;10(1):69-73 PMID: 6250947
  10. Genetic and physical mapping of recF in Escherichia coli K-12.
    Mol Gen Genet. 1980;180(1):115-21 PMID: 6255290
  11. How ribosomes select initiator regions in mRNA: base pair formation between the 3' terminus of 16S rRNA and the mRNA during initiation of protein synthesis in Escherichia coli.
    Proc Natl Acad Sci U S A. 1975 Dec;72(12):4734-8 PMID: 1107998
  12. Nucleotide sequence of Escherichia coli K-12 replication origin.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):575-9 PMID: 370832
  13. Spliced early mRNAs of simian virus 40.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1274-8 PMID: 206891
  14. Organization and transcription of the dnaA and dnaN genes of Escherichia coli.
    Gene. 1981 Jan-Feb;13(1):47-55 PMID: 6453739
  15. Nucleotide sequence of the origin of replication of the Escherichia coli K-12 chromosome.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):580-4 PMID: 370833
  16. Promoter sequence for stringent control of bacterial ribonucleic acid synthesis.
    J Bacteriol. 1980 Feb;141(2):973-6 PMID: 6154042
  17. Cloning and characterization of EcoRI and HindIII restriction endonuclease-generated fragments of antibiotic resistance plasmids R6-5 and R6.
    Mol Gen Genet. 1978 Jun 14;162(2):121-37 PMID: 672900
  18. Coordinate expression of Escherichia coli dnaA and dnaN genes.
    Mol Gen Genet. 1980;179(3):521-6 PMID: 6449652
  19. Replication origin of the Escherichia coli K-12 chromosome: the size and structure of the minimum DNA segment carrying the information for autonomous replication.
    Mol Gen Genet. 1980 Apr;178(1):9-20 PMID: 6991883
  20. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  21. The primary structure of protein L20 from the large subunit of the Escherichia coli ribosome.
    FEBS Lett. 1979 Jul 1;103(1):61-5 PMID: 381019
  22. Simple method for identification of plasmid-coded proteins.
    J Bacteriol. 1979 Jan;137(1):692-3 PMID: 368040
  23. Nucleotide sequence of the ribosomal protein gene cluster adjacent to the gene for RNA polymerase subunit beta in Escherichia coli.
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):1697-701 PMID: 377281
  24. ClaI. a new restriction endonuclease from Caryophanon latum L.
    Nucleic Acids Res. 1981 Oct 10;9(19):4833-45 PMID: 6273788
  25. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  26. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342-6 PMID: 4598299
  27. Origin of replication, oriC, of the Escherichia coli K12 chromosome: genetic mapping and minichromosome replication.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:121-8 PMID: 383376
  28. Recalibrated linkage map of Escherichia coli K-12.
    Bacteriol Rev. 1976 Mar;40(1):116-67 PMID: 773363
  29. The sequence determination of a protein in a micro scale: the sequence analysis of ribosomal protein L34 of Escherichia coli.
    Hoppe Seylers Z Physiol Chem. 1976 Jun;357(6):873-86 PMID: 783033
  30. Bacteriophage T7 early promoters: nucleotide sequences of two RNA polymerase binding sites.
    J Mol Biol. 1975 Dec 15;99(3):419-43 PMID: 765476
  31. The structure of tandem regulatory regions in the deo operon of Escherichia coli K12.
    EMBO J. 1982;1(3):317-22 PMID: 16453417
  32. Ribosomal proteins. 8. Molecular weights of isolated ribosomal proteins of Escherichia coli.
    Proc Natl Acad Sci U S A. 1970 Dec;67(4):1909-13 PMID: 4923120
  33. Conformational alteration of mRNA structure and the posttranscriptional regulation of erythromycin-induced drug resistance.
    Nucleic Acids Res. 1980 Dec 20;8(24):6081-97 PMID: 6162157
  34. Regulation of the dnaA product in Escherichia coli.
    Mol Gen Genet. 1977 Oct 20;155(2):219-25 PMID: 337131
  35. Biological function for 6-methyladenine residues in the DNA of Escherichia coli K12.
    J Mol Biol. 1974 May 15;85(2):309-22 PMID: 4600143
  36. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  37. Genetic analysis of cold-sensitive ribosome maturation mutants of Escherichia coli.
    J Bacteriol. 1974 Mar;117(3):1082-92 PMID: 4591943
  38. Origin of replication, oriC, or the Escherichia coli chromosome on specialized transducing phages lambda asn.
    Mol Gen Genet. 1978 Apr 17;160(3):287-95 PMID: 353492
  39. A temperature-sensitive Escherichia coli mutant defective in DNA replication: dnaN, a new gene adjacent to the dnaA gene.
    Mol Gen Genet. 1980;178(3):541-53 PMID: 6248733
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1982-00-00
Pages
1043-8
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553160
Subset
IM
Databases
GENBANK
J01602
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2