Cloning of the iron superoxide dismutase gene (sodB) in Escherichia coli K-12.
A clone overproducing iron superoxide dismutase has been isolated from an Escherichia coli cosmid bank. Subcloning located the gene responsible for iron superoxide dismutase overproduction on a 6.6-kilobase PstI restriction endonuclease fragment. Maxicell analysis, followed by immunological identification of iron superoxide dismutase protein, demonstrated that the structural gene, sodB, of iron superoxide dismutase has been cloned.
7 references, click to expand
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