Home LiteratureArticle Details
PMID: 6330533 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Enhanced transformation of human cells by UV-irradiated pSV2 plasmids.

Molecular and cellular biology ·Vol. 4 ·No. 6 ·1984-06-00 ·Pages 1169-71

Spivak G, Ganesan AK, Hanawalt PC

Abstract

Irradiating the plasmid pSV2-gpt with UV (254 nm) doses up to 200 J m-2 caused a dose-dependent increase in the yield of Gpt+ transformants when the plasmid was introduced into human cells by calcium phosphate coprecipitation. UV doses greater than 1 kJ m-2 were required to reduce the efficiency of transformation below that obtained with unirradiated DNA.

MeSH Terms
Base Sequence Cell Line Chimera DNA Restriction Enzymes Dose-Response Relationship, Radiation Escherichia coli/enzymology,genetics Fibrosarcoma Humans Pentosyltransferases/genetics Plasmids/radiation effects Transformation, Genetic/radiation effects Ultraviolet Rays Xeroderma Pigmentosum
Chemicals
Pentosyltransferases xanthine phosphoribosyltransferase DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Spivak G
Ganesan A K
Hanawalt P C
References (22)
22 references, click to expand
  1. Inhibition of SV40 replication in simian cells by specific pBR322 DNA sequences.
    Nature. 1981 Sep 3;293(5827):79-81 PMID: 6267479
  2. Regulation of simian virus 40 transcription: sensitive analysis of the RNA species present early in infections by virus or viral DNA.
    J Virol. 1979 Aug;31(2):360-9 PMID: 225559
  3. Characteristics of an SV40-plasmid recombinant and its movement into and out of the genome of a murine cell.
    Cell. 1980 Aug;21(1):127-39 PMID: 6250708
  4. Isolation of supercoiled colicinogenic factor E 1 DNA sensitive to ribonuclease and alkali.
    Proc Natl Acad Sci U S A. 1972 Sep;69(9):2518-22 PMID: 4560690
  5. Improved microfluorometric DNA determination in biological material using 33258 Hoechst.
    Anal Biochem. 1979 Nov 15;100(1):188-97 PMID: 94515
  6. Recombination between temperature-sensitive mutants of simian virus 40.
    Virology. 1974 Jan;57(1):161-74 PMID: 4362021
  7. Antagonists of DNA gyrase inhibit repair and recombination of UV-irradiated phage lambda.
    Proc Natl Acad Sci U S A. 1978 Sep;75(9):4125-9 PMID: 212734
  8. Binding of T4 endonuclease V to deoxyribonucleic acid irradiated with ultraviolet light.
    Biochemistry. 1980 Apr 15;19(8):1685-91 PMID: 6246930
  9. Genetic exchanges caused by ultraviolet photoproducts in phage lambda DNA molecules: the role of DNA replication.
    Mol Gen Genet. 1976 Jul 23;146(2):107-15 PMID: 958200
  10. Expression of a bacterial gene in mammalian cells.
    Science. 1980 Sep 19;209(4463):1422-7 PMID: 6251549
  11. Introduction and recovery of a selectable bacterial gene from the genome of mammalian cells.
    Mol Cell Biol. 1982 Aug;2(8):966-76 PMID: 6290875
  12. Regulated expression of human interferon beta 1 gene after transduction into cultured mouse and rabbit cells.
    Proc Natl Acad Sci U S A. 1982 Sep;79(17):5166-70 PMID: 6957856
  13. Loss of mouse chromosomes in somatic cell hybrids between HT-1080 human fibrosarcoma cells and mouse peritioneal macrophages.
    Proc Natl Acad Sci U S A. 1976 Sep;73(9):3248-52 PMID: 184468
  14. [Genetic study of a temperate bacteriophage of Escherichia coli. III. Effect of ultraviolet rays on genetic recombination].
    Ann Inst Pasteur (Paris). 1955 Jun;88(6):724-49 PMID: 13403339
  15. UV-induced DNA repair synthesis in cells of patients with different forms of xeroderma pigmentosum and of heterozygotes.
    Mutat Res. 1973 Dec;20(3):417-28 PMID: 4778857
  16. Role of host-cell reactivation in the enhancement of complete transduction after UV irradiation of P22 phage.
    Biochem Biophys Res Commun. 1968 Jun 28;31(6):938-44 PMID: 4875731
  17. Procaryotic genomic DNA inhibits mammalian cell transformation.
    Mol Cell Biol. 1983 May;3(5):956-9 PMID: 6865947
  18. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.
    J Mol Appl Genet. 1982;1(4):327-41 PMID: 6286831
  19. Assay for nanogram quantities of DNA in cellular homogenates.
    Anal Biochem. 1979 Jan 15;92(2):497-500 PMID: 87138
  20. A new technique for the assay of infectivity of human adenovirus 5 DNA.
    Virology. 1973 Apr;52(2):456-67 PMID: 4705382
  21. Factors governing the expression of a bacterial gene in mammalian cells.
    Mol Cell Biol. 1981 May;1(5):449-59 PMID: 6100966
  22. Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase.
    Proc Natl Acad Sci U S A. 1981 Apr;78(4):2072-6 PMID: 7017722
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.4.6.1169-1171.1984
Published
1984-06-00
Pages
1169-71
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368887
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.4.6.1169-1171.1984 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2