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PMID: 6330676 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Sequences of the E. coli uvrC gene and protein.

Nucleic acids research ·Vol. 12 ·No. 11 ·1984-06-11 ·Pages 4593-608

Sancar GB, Sancar A, Rupp WD

Abstract

We have determined the sequence of a 2400 bp region of E. coli chromosomal DNA containing the uvrC gene. The coding region of uvrc is 2267 bp in length, encodes a polypeptide with a calculated molecular weight of 66,038 daltons, and is preceded by a typical E. coli ribosome binding site. By constructing deletion derivatives we have established that a uvrC promoter lies within the 113 bp region preceding the translational start of uvrC. The codon usage in uvrC is strongly biased in favor of codons used infrequently in E. coli, which may contribute to the relatively low intracellular concentration of uvrC protein.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Composition Base Sequence Codon/genetics DNA Restriction Enzymes Escherichia coli/genetics,radiation effects Genes Genes, Bacterial Molecular Weight Operon Plasmids Protein Biosynthesis Ultraviolet Rays
Chemicals
Bacterial Proteins Codon DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sancar G B
Sancar A
Rupp W D
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41 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.11.4593
Published
1984-06-11
Pages
4593-608
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318860
Subset
IM
Grants
NCI NIH HHS · CA06519 · United States
NCI NIH HHS · CA09259 · United States
NIGMS NIH HHS · GM24178 · United States
Databases
GENBANK
X00189, X00638, X03691
External Links
PubMed source DOI: 10.1093/nar/12.11.4593 PMC Full Text View journal details
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