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PMID: 6330677 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Antibiotic resistance mutations in 16S and 23S ribosomal RNA genes of Escherichia coli.

Nucleic acids research ·Vol. 12 ·No. 11 ·1984-06-11 ·Pages 4653-63

Sigmund CD, Ettayebi M, Morgan EA

Abstract

Recombinant DNA and classic genetic procedures were used to map a spectinomycin resistance mutation to a 121 base pair region of a 16S RNA gene and a macrolide-lincosamide-streptogramin type B resistance mutation to a 32 base pair region of a 23S RNA gene. DNA sequence analysis of these regions revealed that spectinomycin resistance results from a C/G to T/A transition at position 1192 of a 16S RNA gene. Resistance to macrolide, lincosamide and streptogramin type B antibiotics results from an A/T to T/A transversion at position 2058 of a 23S RNA gene. The alteration in 16S RNA is in a sequence that can participate in alternate base pairing arrangements that have been proposed to be involved in the translocation process. The alteration in 23S RNA identifies sequences important to peptidyl transfer.

MeSH Terms
Base Sequence DNA Restriction Enzymes DNA, Recombinant/analysis Drug Resistance, Microbial Escherichia coli/drug effects,genetics Genes, Bacterial Mutation Nucleic Acid Conformation R Factors RNA, Ribosomal/genetics Spectinomycin/toxicity
Chemicals
DNA, Recombinant RNA, Ribosomal Spectinomycin DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sigmund C D
Ettayebi M
Morgan E A
References (28)
28 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.11.4653
Published
1984-06-11
Pages
4653-63
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318865
Subset
IM
Grants
NIAID NIH HHS · AI-00556 · United States
NIGMS NIH HHS · GM27664 · United States
External Links
PubMed source DOI: 10.1093/nar/12.11.4653 PMC Full Text View journal details
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