Home LiteratureArticle Details
PMID: 6330681 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Transcription of methylated eukaryotic viral genes in a soluble in vitro system.

Nucleic acids research ·Vol. 12 ·No. 11 ·1984-06-11 ·Pages 4715-30

Jove R, Sperber DE, Manley JL

Abstract

SV40 and adenovirus-2 (Ad2) recombinant plasmids containing long segments of poly(dC-dG) cloned adjacent to transcription control regions were methylated in vitro with Hbal methylase and transcribed in a soluble in vitro system. The addition of up to 40 or more base pairs of poly(m5dC-dG) immediately upstream or downstream of promoter regions was shown to have no effect on the accuracy or efficiency of specific transcription from these promoters in vitro. Methylation at various naturally occurring C-G sequences within or near these promoters also had no effect on transcription in vitro. The significance of these results with respect to possible mechanisms whereby DNA methylation might regulate eukaryotic gene expression is discussed.

MeSH Terms
Adenoviruses, Human/genetics Base Composition Base Sequence Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant/metabolism Deoxyribonucleases, Type II Site-Specific Genes, Viral HeLa Cells/metabolism Humans Operon Plasmids Polydeoxyribonucleotides Simian virus 40/genetics Transcription, Genetic
Chemicals
DNA, Recombinant Polydeoxyribonucleotides poly(dC-dG) DNA Restriction Enzymes Deoxyribonucleases, Type II Site-Specific GCGC-specific type II deoxyribonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Jove R
Sperber D E
Manley J L
References (39)
39 references, click to expand
  1. DNA methylation in the human gamma delta beta-globin locus in erythroid and nonerythroid tissues.
    Cell. 1980 Apr;19(4):947-58 PMID: 6247075
  2. DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3855-9 PMID: 6933441
  3. DNA methylation and gene function.
    Science. 1980 Nov 7;210(4470):604-10 PMID: 6254144
  4. Transcriptional control regions of the adenovirus VAI RNA gene.
    Cell. 1980 Nov;22(2 Pt 2):405-13 PMID: 7448868
  5. Transcription of Simian virus 40 DNA in a HeLa whole cell extract.
    J Biol Chem. 1981 Jan 10;256(1):478-82 PMID: 6256354
  6. Left-handed DNA in restriction fragments and a recombinant plasmid.
    Nature. 1981 Apr 23;290(5808):672-7 PMID: 6261149
  7. DNA sequence required for initiation of transcription in vitro from the major late promoter of adenovirus 2.
    Proc Natl Acad Sci U S A. 1981 Feb;78(2):820-4 PMID: 6262775
  8. Effects of methylation on a synthetic polynucleotide: the B--Z transition in poly(dG-m5dC).poly(dG-m5dC).
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1619-23 PMID: 6262820
  9. The somatic replication of DNA methylation.
    Cell. 1981 Apr;24(1):33-40 PMID: 6263490
  10. Chromatin structure of endogenous retroviral genes and activation by an inhibitor of DNA methylation.
    Nature. 1981 Jul 23;292(5821):311-7 PMID: 6166864
  11. Antibodies to left-handed Z-DNA bind to interband regions of Drosophila polytene chromosomes.
    Nature. 1981 Dec 3;294(5840):417-22 PMID: 6796893
  12. Expression of a cloned adenovirus gene is inhibited by in vitro methylation.
    Proc Natl Acad Sci U S A. 1982 Feb;79(4):1073-7 PMID: 6951163
  13. In vitro methylation of the hamster adenine phosphoribosyltransferase gene inhibits its expression in mouse L cells.
    Proc Natl Acad Sci U S A. 1982 Jun;79(11):3418-22 PMID: 6954487
  14. Flipping of cloned d(pCpG)n.d(pCpG)n DNA sequences from right- to left-handed helical structure by salt, Co(III), or negative supercoiling.
    Proc Natl Acad Sci U S A. 1982 Aug;79(15):4560-4 PMID: 6956879
  15. Methylation of simian virus 40 Hpa II site affects late, but not early, viral gene expression.
    Proc Natl Acad Sci U S A. 1982 Sep;79(17):5142-6 PMID: 6291026
  16. Undermethylation at the 5' end of the albumin gene is necessary but not sufficient for albumin production by rat hepatoma cells in culture.
    Cell. 1982 Oct;30(3):825-33 PMID: 7139715
  17. A novel repeated element with Z-DNA-forming potential is widely found in evolutionarily diverse eukaryotic genomes.
    Proc Natl Acad Sci U S A. 1982 Nov;79(21):6465-9 PMID: 6755470
  18. DNA-protein complexes spread on N2-discharged carbon film and characterized by molecular weight and its projected distribution.
    J Mol Biol. 1982 Sep 15;160(2):375-86 PMID: 6757448
  19. Negatively supercoiled plasmids contain left-handed Z-DNA segments as detected by specific antibody binding.
    Cell. 1982 Dec;31(2 Pt 1):309-18 PMID: 7159926
  20. Site-specific base substitution and deletion mutations that enhance or suppress transcription of the SV40 major late RNA.
    Cell. 1982 Dec;31(3 Pt 2):625-33 PMID: 7159930
  21. Z-DNA immunoreactivity in rat tissues.
    Nature. 1983 Jun 9-15;303(5917):540-3 PMID: 6343886
  22. Methylation of milk-borne and genetically transmitted mouse mammary tumor virus proviral DNA.
    Cell. 1980 Mar;19(3):653-62 PMID: 6244894
  23. Negatively supercoiled simian virus 40 DNA contains Z-DNA segments within transcriptional enhancer sequences.
    Nature. 1983 Jun 23-29;303(5919):674-9 PMID: 6304535
  24. DNA methylation and the regulation of globin gene expression.
    Cell. 1983 Aug;34(1):197-206 PMID: 6883509
  25. In vitro transcription: whole-cell extract.
    Methods Enzymol. 1983;101:568-82 PMID: 6193397
  26. DNA methylation and gene activity.
    Annu Rev Biochem. 1983;52:93-124 PMID: 6311083
  27. Complete DNA methylation does not prevent polyoma and simian virus 40 virus early gene expression.
    Proc Natl Acad Sci U S A. 1983 Nov;80(21):6470-4 PMID: 6314328
  28. Transcription in oocytes of highly methylated rDNA from Xenopus laevis sperm.
    Nature. 1983 Nov 10-16;306(5939):200-3 PMID: 6646203
  29. Developmental activation of the H-2K gene is correlated with an increase in DNA methylation.
    Cell. 1983 Dec;35(2 Pt 1):457-65 PMID: 6197180
  30. Expression of the chloramphenicol acetyltransferase gene in mammalian cells under the control of adenovirus type 12 promoters: effect of promoter methylation on gene expression.
    Proc Natl Acad Sci U S A. 1983 Dec;80(24):7586-90 PMID: 6324179
  31. A specific endonuclease from Haemophilus haemolyticus.
    J Mol Biol. 1976 May 5;103(1):199-208 PMID: 1085372
  32. Specificity of Hpa II and Hae III DNA methylases.
    Nucleic Acids Res. 1977 Dec;4(12):4211-21 PMID: 600794
  33. Chromatin assembly and transcription in eggs and oocytes of Xenopus laevis.
    Cold Spring Harb Symp Quant Biol. 1978;42 Pt 1:171-8 PMID: 209936
  34. Specific DNA methylation sites in the vicinity of the chicken beta-globin genes.
    Nature. 1979 Aug 2;280(5721):419-20 PMID: 460418
  35. Multiple new phenotypes induced in 10T1/2 and 3T3 cells treated with 5-azacytidine.
    Cell. 1979 Aug;17(4):771-9 PMID: 90553
  36. Methylation of Herpesvirus saimiri DNA in lymphoid tumor cell lines.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3839-43 PMID: 226983
  37. Molecular structure of a left-handed double helical DNA fragment at atomic resolution.
    Nature. 1979 Dec 13;282(5740):680-6 PMID: 514347
  38. DNA methylation: organ specific variations in the methylation pattern within and around ovalbumin and other chicken genes.
    Nucleic Acids Res. 1979 Dec 20;7(8):2081-103 PMID: 523314
  39. Methylation of integrated adenovirus type 12 DNA sequences in transformed cells is inversely correlated with viral gene expression.
    Proc Natl Acad Sci U S A. 1980 Jan;77(1):253-6 PMID: 6244548
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.11.4715
Published
1984-06-11
Pages
4715-30
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318870
Subset
IM
Grants
NCI NIH HHS · CA33620 · United States
External Links
PubMed source DOI: 10.1093/nar/12.11.4715 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2