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PMID: 6333426 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Leukocyte interleukins induce cultured endothelial cells to produce a highly organized, glycosaminoglycan-rich pericellular matrix.

The Journal of cell biology ·Vol. 99 ·No. 5 ·1984-11-00 ·Pages 1706-15

Montesano R, Mossaz A, Ryser JE, Orci L, Vassalli P

Abstract

We report here that interleukins have a dramatic effect on extracellular matrix production by cultured endothelial cells. Human umbilical vein endothelial cells incubated with growth media conditioned by lectin-activated human peripheral blood mononuclear leukocytes undergo marked changes in cell shape and elaborate a highly organized extracellular material that is not detectable in untreated cultures. This material has the following characteristics: (a) it is not recognizable by electron microscopy unless the cationic dye, Alcian blue, is added to the fixative; (b) it is visualized as a network of branching and anastomosing fibrils of various thickness that can be resolved into bundles of fine filaments; (c) it is associated with the cell surface, extends between contiguous cells, and coats the culture substrate; (d) it is removed by digestion with glycosaminoglycan-degrading enzymes, such as crude heparinase and chondroitinase ABC. These results demonstrate that soluble factors released by activated peripheral blood mononuclear leukocytes (interleukins) stimulate cultured human umbilical vein endothelial cells to produce a highly structured pericellular matrix containing glycosaminoglycans (probably chondroitin sulfate and/or hyaluronic acid) as a major constituent. We speculate that this phenomenon corresponds to an early step of angiogenesis as observed in vivo as a consequence of interleukin release.

MeSH Terms
Alcian Blue Animals Cells, Cultured Chondroitin Lyases/pharmacology Endothelium/physiology Extracellular Matrix/drug effects,metabolism,ultrastructure Glucuronidase Glycosaminoglycans/metabolism Glycoside Hydrolases/pharmacology Humans Interleukin-2/physiology Leukocytes/physiology Microscopy, Electron Phytohemagglutinins/pharmacology Umbilical Veins
Chemicals
Glycosaminoglycans Interleukin-2 Phytohemagglutinins Glycoside Hydrolases heparanase Glucuronidase Chondroitin Lyases Alcian Blue
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Montesano R
Mossaz A
Ryser J E
Orci L
Vassalli P
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48 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.99.5.1706
Published
1984-11-00
Pages
1706-15
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2113367
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.99.5.1706 PMC Full Text View journal details
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