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PMID: 6334854 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Human interleukin 2 can promote the growth and differentiation of single hapten-specific B cells in the presence of specific antigen.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 24 ·1984-12-00 ·Pages 7917-21

Pike BL, Raubitschek A, Nossal GJ

Abstract

B lymphocytes specifically reactive to the hapten fluorescein (FLU) were prepared from normal adult murine spleen by the hapten-gelatin affinity procedure. They were placed in 10 microliter of microcultures singly or in small numbers in the absence of any feeder, filler, or accessory cell. The "T cell-independent" antigen FLU-conjugated polymerized flagellin (FLU-POL) or a selected batch of FLU-conjugated Ficoll were used, and these stimulated division and differentiation only in the concomitant presence of lymphokines acting as B-cell growth and differentiation factors (BGDF). It was found that human interleukin 2 (IL-2), prepared by recombinant DNA technology (r-IL-2), was an effective, albeit rather weak, BGDF in this system. When an IL-2-free source of BGDF was used with the antigenic stimulus, addition of r-IL-2 did not augment the response, nor did removal of IL-2 from the crude lymphokine mixture diminish the BGDF activity.

MeSH Terms
Animals B-Lymphocytes/cytology,drug effects,immunology Cell Differentiation/drug effects Cell Line Growth Substances/immunology Haptens Humans Interleukin-2/pharmacology Interleukin-4 Kinetics Lymphocyte Activation/drug effects Lymphokines/immunology Mice Mice, Inbred Strains Thymoma/immunology Thymus Neoplasms/immunology
Chemicals
Growth Substances Haptens Interleukin-2 Lymphokines Interleukin-4
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Pike B L
Raubitschek A
Nossal G J
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29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.24.7917
Published
1984-12-00
Pages
7917-21
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC392264
Subset
IM
Grants
NIAID NIH HHS · AI-03958 · United States
External Links
PubMed source DOI: 10.1073/pnas.81.24.7917 PMC Full Text View journal details
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