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PMID: 6339371 Published · ppublish English Case Reports Journal Article

Characterization of a new human endometrial carcinoma (RL95-2) established in tissue culture.

In vitro ·Vol. 19 ·No. 3 Pt 1 ·1983-03-00 ·Pages 147-58

Way DL, Grosso DS, Davis JR, Surwit EA, Christian CD

Abstract

A new human endometrial cell line, RL95-2, derived from a Grade 2 moderately differentiated adenosquamous carcinoma of the endometrium has been passaged successfully in cell culture for more than 2 yr. The cells are characteristically epithelioid with well-defined junctional complexes, tonofilaments, filopodialike extensions, and surface microvilli. Nuclei are large, irregular, and invaginated frequently with multiple, prominent, lamellar nucleoli. The cells have a log phase doubling time of 22 to 34 h followed by continued growth at a reduced rate with no apparent plateau phase. They exhibit a strong tendency for piling up as well as for the formation of glandlike dome structures. Karyotypically the line is trisomic 8 (47,XX,+8) and has an 8% frequency of polyploidization. Both cytoplasmic and nuclear estrogen receptors are present. Antihuman alpha-keratin characterizes the cell line as epithelial, nonstromal. The RL95-2 cell line may provide a useful in vitro system for the investigation of the endocrine regulation of endometrial neoplasia.

MeSH Terms
Adenocarcinoma/pathology,ultrastructure Aged Carcinoma, Squamous Cell/pathology,ultrastructure Cell Line Culture Techniques Cytoplasm/metabolism Female Fluorescent Antibody Technique Humans Karyotyping Microscopy, Electron, Scanning Receptors, Estrogen/analysis Uterine Neoplasms/metabolism,pathology,ultrastructure
Chemicals
Receptors, Estrogen
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Way D L
Grosso D S
Davis J R
Surwit E A
Christian C D
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Article Info
Journal
In vitro
Abbr.
In Vitro
ISSN
0073-5655
DOI
10.1007/BF02618053
Published
1983-03-00
Pages
147-58
Language
English
Region
United States
NLM ID
0063733
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02618053
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