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PMID: 6341242 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Inhibition of enterotoxin from Escherichia coli and Vibrio cholerae by gangliosides from human milk.

Infection and immunity ·Vol. 40 ·No. 2 ·1983-05-00 ·Pages 563-9

Otnaess AB, Laegreid A, Ertresvåg K

Abstract

Inhibitory activity of enterotoxin from Escherichia coli and Vibrio cholerae was associated with the ganglioside fraction of human milk. Both the milk fat and skim milk contained gangliosides that inhibited the toxins. The most purified milk fraction contained three glycolipid components, of which two migrated close to ganglioside GM1 on thin-layer chromatography plates. A component with a slightly different mobility from GM1 appeared to be associated with the inhibitory activity. Milk ganglioside fraction, derived from 2 ml of human milk, contained 1 to 4 micrograms of lipid-bound sialic acid and completely inhibited 0.1 micrograms of cholera toxin in rabbit intestinal loop experiments. It is suggested that human milk gangliosides, although present only in trace amounts, may be important in protecting infants against enterotoxin-induced diarrhea.

MeSH Terms
Enterotoxins/antagonists & inhibitors Escherichia coli/immunology Female G(M1) Ganglioside/pharmacology Gangliosides/pharmacology Humans Lipids/pharmacology Milk, Human/immunology Vibrio cholerae/immunology
Chemicals
Enterotoxins Gangliosides Lipids G(M1) Ganglioside
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Otnaess A B
Laegreid A
Ertresvåg K
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29 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.40.2.563-569.1983
Published
1983-05-00
Pages
563-9
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC264892
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.40.2.563-569.1983 PMC Full Text View journal details
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