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PMID: 6341817 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The secreted form of invertase in Saccharomyces cerevisiae is synthesized from mRNA encoding a signal sequence.

Molecular and cellular biology ·Vol. 3 ·No. 3 ·1983-03-00 ·Pages 439-47

Carlson M, Taussig R, Kustu S, Botstein D

Abstract

The SUC2 gene of Saccharomyces cerevisiae encodes two differently regulated mRNAs (1.8 and 1.9 kilobases) that differ at their 5' ends. The larger RNA encodes a secreted, glycosylated form of invertase and the smaller RNA encodes an intracellular, nonglycosylated form. We have determined the nucleotide sequence of the amino-terminal coding region of the SUC2 gene and its upstream flanking region and have mapped the 5' ends of the SUC2 mRNAs relative to the DNA sequence. The 1.9-kilobase RNA contains a signal peptide coding sequence and presumably encodes a precursor to secreted invertase. The 1.8-kilobase RNA does not include the complete coding sequence for the signal peptide. The nucleotide sequence data prove that SUC2 is a structural gene for invertase, and translation of the coding information provides the complete amino acid sequence of an S. cerevisiae signal peptide.

MeSH Terms

Amino Acid Sequence Base Sequence Genes Glycoside Hydrolases/biosynthesis,genetics RNA, Messenger/metabolism Saccharomyces cerevisiae/enzymology beta-Fructofuranosidase

Chemicals

RNA, Messenger Glycoside Hydrolases beta-Fructofuranosidase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Carlson M

Taussig R

Kustu S

Botstein D

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26 references, click to expand

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Article Info

Journal

Molecular and cellular biology

Abbr.

Mol Cell Biol

ISSN

0270-7306

DOI

10.1128/mcb.3.3.439-447.1983

Published

1983-03-00

Pages

439-47

Language

English

Region

United States

NLM ID

8109087

PMCID

PMC368553

Subset

Databases

GENBANK

K00540, V01311

External Links

PubMed source DOI: 10.1128/mcb.3.3.439-447.1983 PMC Full Text View journal details

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The secreted form of invertase in Saccharomyces cerevisiae is synthesized from mRNA encoding a signal sequence.

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