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PMID: 6344791 Published · ppublish English Comparative Study Journal Article

Characteristics of Escherichia coli grown in bay water as compared with rich medium.

Applied and environmental microbiology ·Vol. 45 ·No. 4 ·1983-04-00 ·Pages 1316-23

Chai TJ

Abstract

Membrane-filtered bay water can support a certain degree of growth of Escherichia coli organisms isolated from the bay water or from sewage. The effect of the growth medium (bay water versus rich medium) on sensitivities to antimicrobial agents and cell envelope proteins was studied in many of these strains. Bay water-grown cells were less sensitive to bacteriophages and colicins, but were more sensitive to heavy metals and detergents as compared with rich-medium-grown cells. These results indicated that the cell envelope composition of the bay water-grown cells could be modified, resulting in altered susceptibility to various antimicrobial agents. An analysis of cell envelope proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that cells from rich-medium-grown cultures contained two or three major outer membrane proteins, whereas in bay water-grown cells, the OmpF protein was greatly reduced.

MeSH Terms
Bacterial Outer Membrane Proteins Bacterial Proteins/analysis Cell Membrane/analysis Colicins/pharmacology Coliphages/physiology Culture Media Detergents/pharmacology Escherichia coli/analysis,physiology Membrane Proteins/analysis Metals/pharmacology Seawater Water Microbiology
Chemicals
Bacterial Outer Membrane Proteins Bacterial Proteins Colicins Culture Media Detergents Membrane Proteins Metals
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Chai T J
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28 references, click to expand
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.45.4.1316-1323.1983
Published
1983-04-00
Pages
1316-23
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC242457
Subset
IM
External Links
PubMed source DOI: 10.1128/aem.45.4.1316-1323.1983 PMC Full Text View journal details
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