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PMID: 6345513 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Lactoperoxidase and Iodo-Gen-catalyzed iodination labels inner and outer membrane proteins of Haemophilus influenzae.

Journal of bacteriology ·Vol. 155 ·No. 1 ·1983-07-00 ·Pages 443-6

Loeb MR, Smith DH

Abstract

Both inner and outer membrane proteins of Haemophilus influenzae type b were labeled by iodination procedures believed to be specific for exposed surface proteins only. It is suggested that this is due to specific properties of the outer membrane of H. influenzae and that use of these procedures with other gram-negative bacteria be evaluated carefully.

MeSH Terms

Carbon Radioisotopes Cell Membrane/analysis Electrophoresis, Polyacrylamide Gel Haemophilus influenzae/analysis Iodine Radioisotopes Lactoperoxidase Membrane Proteins/analysis Radioisotope Dilution Technique

Chemicals

Carbon Radioisotopes Iodine Radioisotopes Membrane Proteins Lactoperoxidase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Loeb M R

Smith D H

References (14)

14 references, click to expand

Exposed protein on the intact human erythrocyte.
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Amplification of the lactose carrier protein in Escherichia coli using a plasmid vector.
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The surface properties of Neisseria gonorrhoeae: topographical distribution of the outer membrane protein antigens.
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Surface-specific iodination of membrane proteins of viruses and eucaryotic cells using 1,3,4,6-tetrachloro-3alpha,6alpha-diphenylglycoluril.
Biochemistry. 1978 Oct 31;17(22):4807-17 PMID: 215191
Outer membrane of Escherichia coli: properties of the F sex factor traT protein which is involved in surface exclusion.
J Bacteriol. 1980 Apr;142(1):285-94 PMID: 6989806
Lactoperoxidase-catalyzed iodination as a tool for investigation of proteins.
Methods Enzymol. 1980;70(A):214-20 PMID: 7421590
Isolation and partial characterization of outer and inner membranes from encapsulated Haemophilus influenzae type b.
J Bacteriol. 1981 Jan;145(1):596-604 PMID: 6970193
Identification of immunogenic outer membrane proteins of Haemophilus influenzae type b in the infant rat model system.
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Structural and functional evidence of cooperativity between membranes and cell wall in bacteria.
Int Rev Cytol Suppl. 1981;12:39-70 PMID: 7019122
Haemophilus influenzae polypeptides involved in deoxyribonucleic acid uptake detected by cellular surface protein iodination.
J Bacteriol. 1981 Oct;148(1):220-31 PMID: 6974728
Use of iodo-gen and iodine-125 to label the outer membrane proteins of whole cells of Neisseria gonorrhoeae.
Anal Biochem. 1982 Mar 1;120(2):254-8 PMID: 6807134
Effects of nonionic, ionic, and dipolar ionic detergents and EDTA on the Brucella cell envelope.
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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.155.1.443-446.1983

Published

1983-07-00

Pages

443-6

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC217703

Subset

Grants

NIAID NIH HHS · AI-14411 · United States

External Links

PubMed source DOI: 10.1128/jb.155.1.443-446.1983 PMC Full Text View journal details

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Lactoperoxidase and Iodo-Gen-catalyzed iodination labels inner and outer membrane proteins of Haemophilus influenzae.

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