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PMID: 634793 Published · ppublish English Journal Article

Isolation from rat liver and sequence of a RNA fragment containing 32 nucleotides from position 5 to 36 from the 3' end of ribosomal 18S RNA.

Nucleic acids research ·Vol. 5 ·No. 2 ·1978-02-00 ·Pages 425-34

Alberty H, Raba M, Gross HJ

Abstract

Crude tRNA isolated from rat liver by the method of Rogg et al. (Biochem. Biophys. Acta 195, 13-15 1969) contains N6-dimethyladenosine (m6-2A) and was therefore fractionated in order to identify the m6-2A-containing RNAs. A unique species of RNA was purified which contained all the m62A present in the crude tRNA. Sequence analysis by postlabeling with gamma-32p-ATP and polynucleotide kinase revealed that this RNA represents the 32 nucleotides AAGGUUUC(C)U GUAGGUGm62Am62ACCUGCGGAAGGAUC from position 5 to 36 of the 3' terminus of ribosomal 18S RNA. The 36 nucleotide long sequence from the 3' end of rat liver 18S rRNA exhibits extensive homology with the corresponding sequence of E. coli 16S rRNA and with the 21 nucleotide long 3' terminal sequence so far known from Saccharomyces carlsbergensis 17S rRNA. A heterogeneity in this sequence provides the first evidence on the molecular level for the existence of (at least) two sets of redundant ribosomal 18S RNA genes in the rat.

MeSH Terms
Adenine Nucleotides/analysis Animals Base Sequence Liver/analysis Molecular Weight RNA, Ribosomal/analysis,isolation & purification Rats
Chemicals
Adenine Nucleotides RNA, Ribosomal
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Alberty H
Raba M
Gross H J
References (28)
28 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/5.2.425
Published
1978-02-00
Pages
425-34
Language
English
Region
England
NLM ID
0411011
PMCID
PMC341992
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/5.2.425 PMC Full Text View journal details
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