Superstructure and CD spectrum as probes of chromatin integrity.

Nucleic acids research ·Vol. 5 ·No. 2 ·1978-02-00 ·Pages 523-35

de Murcia G, Das GC, Erard M, Daune M

Abstract

Two types of chromatin were extracted from the same stock of rat liver nuclei by a short exposure to micrococcal nuclease and by shearing respectively. These two materials which are identical in their protein/DNA content and by the presence of the five histones, were compared by means of circular dichroism and electron microscopy. Under the electron microscope and in absence of any divalent cation a superstructure of the unfixed chromatin fiber can be viewed only with native material but is no more present in sheared one. The increase of CD signal at 280 nm (from 2000 to about 4000 cm2 deg.dmole-1) in the case of sheared chromatin is not related to the loss of superstructure but to the structural changes of DNA inside the nucleosomal core which are always produced by shearing. These two correlated observations offer new sensitive probes of the integrity of any native or reconstituted chromatin.

MeSH Terms

Animals Cell Nucleus/ultrastructure Chromatin/analysis,isolation & purification,ultrastructure Circular Dichroism Histones/analysis Liver/ultrastructure Micrococcal Nuclease/metabolism Microscopy, Electron Nucleic Acid Denaturation Rats Spectrophotometry, Ultraviolet

Chemicals

Chromatin Histones Micrococcal Nuclease

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

de Murcia G

Das G C

Erard M

Daune M

References (27)

27 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/5.2.523

Published

1978-02-00

Pages

523-35

Language

English

Region

England

NLM ID

0411011

PMCID

PMC341999

Subset

External Links

PubMed source DOI: 10.1093/nar/5.2.523 PMC Full Text View journal details

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Superstructure and CD spectrum as probes of chromatin integrity.

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