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PMID: 6348022 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Amplifiable resistance to tetracycline, chloramphenicol, and other antibiotics in Escherichia coli: involvement of a non-plasmid-determined efflux of tetracycline.

Journal of bacteriology ·Vol. 155 ·No. 2 ·1983-08-00 ·Pages 531-40

George AM, Levy SB

Abstract

Increasing levels of resistance to tetracycline and to a number of other unrelated antibiotics, including chloramphenicol, beta-lactams, puromycin, and nalidixic acid, occurred in Escherichia coli after 50 to 200 generations of growth in the presence of subinhibitory concentrations of tetracycline or chloramphenicol. In the absence of selective pressure, resistances fell to low levels within 100 generations of growth. This amplification of resistance was observed in laboratory and naturally occurring E. coli strains as well as in polA and recA strains. With the exception of previously identified cmlA and cmlB mutations, tetracycline or chloramphenicol resistances were not P1 transducible. Coincident with the emergence of resistance was the appearance of a previously cryptic energy-dependent efflux system for tetracycline. The expression of resistance phenotypes and the tetracycline efflux system were temperature sensitive at 42 degrees C.

MeSH Terms
Anti-Bacterial Agents/metabolism,pharmacology Biological Transport, Active Chloramphenicol/pharmacology Drug Resistance, Microbial Escherichia coli/drug effects,genetics,growth & development Fusaric Acid/pharmacology Gene Amplification Phenotype Temperature Tetracycline/pharmacology Transduction, Genetic
Chemicals
Anti-Bacterial Agents Chloramphenicol Tetracycline Fusaric Acid
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
George A M
Levy S B
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32 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.155.2.531-540.1983
Published
1983-08-00
Pages
531-40
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC217720
Subset
IM
Grants
NIAID NIH HHS · AI16756 · United States
External Links
PubMed source DOI: 10.1128/jb.155.2.531-540.1983 PMC Full Text View journal details
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