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PMID: 6348474 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Two pathways of division inhibition in UV-irradiated E. coli.

Molecular & general genetics : MGG ·Vol. 190 ·No. 2 ·1983-00-00 ·Pages 309-14

Burton P, Holland IB

Abstract

We have investigated the mechanism of division inhibition in E. coli following UV-irradiation or nalidixic acid treatment. After UV, two separate mechanisms, both dependent upon recA+, appear to block division. One mechanism is dependent upon sfiA and sfiB, is inhibited by low levels (4 micrograms/ml) of rifamycin and is expressed in tif mutants at 42 degrees C. The second mechanism is independent of sfiA, and sfiB, is resistant to rifamycin and does not occur in cells lacking DNA replication forks. We suggest that this second mechanism is the result of the failure to terminate DNA replication in inhibited cells. Nalidixic acid inhibition of cell division also appears to involve both mechanisms but as found previously replication forks are also necessary to induce the sfi pathway.

MeSH Terms
Bacterial Proteins/metabolism Cell Division/drug effects,radiation effects DNA Replication DNA, Bacterial/radiation effects Escherichia coli/drug effects,radiation effects Genes, Bacterial Nalidixic Acid/pharmacology Rec A Recombinases Rifampin/pharmacology Ultraviolet Rays
Chemicals
Bacterial Proteins DNA, Bacterial Nalidixic Acid Rec A Recombinases Rifampin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Burton P
Holland I B
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21 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00330656
Published
1983-00-00
Pages
309-14
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00330656
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