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PMID: 6348704 Published · ppublish English Journal Article

Different levels of induction of RecA protein in E. coli (PQ 10) after treatment with two related carcinogens.

Nucleic acids research ·Vol. 11 ·No. 15 ·1983-08-11 ·Pages 5235-42

Salles B, Lang MC, Freund AM, Paoletti C, Daune M, Fuchs RP

Abstract

By means of an immunoradiometric assay the induction of protein RecA in E. coli PQ 10 was measured after treatment by two related carcinogens. On an adduct basis N-Acetoxy-N-2-acetylaminofluorene was shown to induce the protein RecA at a similar level as U.V. On the other hand, N-hydroxy-N-2-aminofluorene shows only a poor induction capacity of the RecA protein. The difference in the SOS inducing potential of the aminofluorene and acetylaminofluorene adducts is discussed in relation to the major difference in the local conformational change the two adducts induce in DNA.

MeSH Terms
2-Acetylaminofluorene/analogs & derivatives Acetoxyacetylaminofluorene/toxicity Bacterial Proteins/genetics Carcinogens/toxicity DNA, Bacterial/genetics Escherichia coli/drug effects,genetics,radiation effects Fluorenes/toxicity Kinetics Rec A Recombinases Ultraviolet Rays
Chemicals
Bacterial Proteins Carcinogens DNA, Bacterial Fluorenes Acetoxyacetylaminofluorene N-hydroxy-2-aminofluorene 2-Acetylaminofluorene Rec A Recombinases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Salles B
Lang M C
Freund A M
Paoletti C
Daune M
Fuchs R P
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21 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.15.5235
Published
1983-08-11
Pages
5235-42
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326256
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/11.15.5235 PMC Full Text View journal details
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