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PMID: 6350184 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Postphagocytic detection of glycopeptidolipids associated with the superficial L1 layer of Mycobacterium intracellulare.

Infection and immunity ·Vol. 41 ·No. 3 ·1983-09-00 ·Pages 1312-21

Tereletsky MJ, Barrow WW

Abstract

The superficial L1 layer of Mycobacterium intracellulare serovar 20 was detected by immunocytochemical procedures based on C-mycoside glycopeptidolipid (GPL) antigens. Specific immune serum was produced by injecting a GPL-methylated bovine serum albumin complex into rabbits with Freund incomplete adjuvant. The resulting antibodies were used to label serovar 20 with goat anti-rabbit immunoglobulin G (IgG) conjugated with either fluorescein or ferritin. The labeled GPL antigens were then detected by fluorescent and electron microscopy. With these procedures, it was possible to observe the superficial distribution of the GPL antigens on serovar 20 and to confirm their intraphagosomal location within the macrophages after phagocytosis. These immunocytochemical procedures now make it possible to monitor these mycobacterial antigens during phagocytosis and may be helpful in determining their role in pathogenesis.

MeSH Terms
Animals Antigens, Bacterial/analysis Antigens, Surface/analysis Female Ferritins/immunology Fluorescent Antibody Technique Glycolipids/analysis,immunology Glycopeptides/analysis,immunology Immunochemistry Macrophages/immunology Male Mice Mice, Inbred C57BL Mycobacterium/immunology Nontuberculous Mycobacteria/immunology Phagocytosis Rabbits
Chemicals
Antigens, Bacterial Antigens, Surface Glycolipids Glycopeptides Ferritins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tereletsky M J
Barrow W W
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.41.3.1312-1321.1983
Published
1983-09-00
Pages
1312-21
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC264641
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.41.3.1312-1321.1983 PMC Full Text View journal details
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