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PMID: 6358236 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Epinemin: a new protein associated with vimentin filaments in non-neural cells.

The Journal of cell biology ·Vol. 97 ·No. 6 ·1983-12-00 ·Pages 1891-905

Lawson D

Abstract

In this report I describe a new protein, defined by a monoclonal antibody, which is associated with vimentin filaments in a variety of cultured cells and in skeletal muscle. By immunofluorescence it is absent in smooth muscle, in cells without vimentin, and in neural vimentin containing cells. This protein has a molecular weight of 44,500, a pl of 5, a two-dimensional tryptic peptide fingerprint pattern different from vimentin, is unrelated to actin by Cleveland peptide analysis and by light and electron microscopy, and is not recognized by either a polyclonal antivimentin antibody (Frank, E.D., and L. Warren, 1981, Proc. Natl. Acad. Sci. USA, 78:3020-3024) or a monoclonal antibody against all classes of intermediate filaments (Pruss, R.M., R. Mirsky, M.C. Raff, R. Thorpe, A.J. Dowding, and B.H. Anderton, 1981, Cell, 27:419-428). The protein is resistant to nonionic detergent extraction, is soluble in high salt and can thus be removed from vimentin filaments, but fragments with vimentin in either low salt or anionic detergent and collapses with vimentin in colchicine-treated cells. By light microscopy, the distribution of the protein is indistinguishable from vimentin filaments and appears uniform along them. In contrast, immunoferritin electron microscopy reveals that the molecule is distributed in an intermittent pattern on vimentin filaments. Adopting the terminology of Granger and Lazarides (1980, Cell, 30:263-275), the molecule is called epinemin, meaning "upon filaments."

MeSH Terms
Animals Antibodies, Monoclonal Cell Line Cells, Cultured Chickens Chlorocebus aethiops Colchicine/pharmacology Cytochalasin D Cytochalasins/pharmacology Cytoskeleton/drug effects,ultrastructure Electrophoresis, Polyacrylamide Gel Fluorescent Antibody Technique Gizzard, Avian/analysis Intermediate Filament Proteins/isolation & purification Kidney Mice Muscles/ultrastructure Peptide Fragments/analysis Vimentin
Chemicals
Antibodies, Monoclonal Cytochalasins Intermediate Filament Proteins Peptide Fragments Vimentin epinemin Cytochalasin D Colchicine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Lawson D
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49 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.97.6.1891
Published
1983-12-00
Pages
1891-905
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2112719
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.97.6.1891 PMC Full Text View journal details
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