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PMID: 6358799 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

A comparison of the origin of replication of pSa with R6K.

Molecular & general genetics : MGG ·Vol. 192 ·No. 1-2 ·1983-00-00 ·Pages 32-8

Tait RC, Kado CI, Rodriguez RL

Abstract

The plasmid pOri3 is a derivative of the origin of replication of pSa. Replication is defective as a result of a truncated repA gene, the product of which is required for plasmid replication. The defective replication is complemented by the presence of the intact repA gene of pSa, or by the presence of the plasmid R6K. The basis of this complementation has been examined by comparing the nucleotide sequence of the origin of pSa with that of R6K. A 13 base pair sequence present twice in the origin of pSa has homology with a 13 base pair sequence that is present fourteen times in the origin of R6K. These sequences may be the binding sites for the initiator proteins of these two plasmids. The location of these binding sites relative to the genes for the initiator proteins suggests that an autoregulatory control mechanism for the synthesis of the initiator proteins may also play a role in the control of plasmid copy number.

MeSH Terms
Base Sequence DNA Replication Escherichia coli/genetics Genes, Bacterial Genetic Complementation Test Plasmids Replicon
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tait R C
Kado C I
Rodriguez R L
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23 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00327643
Published
1983-00-00
Pages
32-8
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NCI NIH HHS · CA-11526 · United States
NIGMS NIH HHS · GM29100-02 · United States
Databases
GENBANK
X00060, X00061
External Links
PubMed source DOI: 10.1007/BF00327643
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