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PMID: 6358800 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

A novel type of E. coli mutants with increased chromosomal copy number.

Molecular & general genetics : MGG ·Vol. 192 ·No. 1-2 ·1983-00-00 ·Pages 51-60

Tanaka M, Ohmori H, Hiraga S

Abstract

We have isolated E. coli mutants which can grow at 30 degrees C but not at 42 degrees C and are able to harbor the oriC plasmid (minichromosome) at a higher copy number than the parental wild-type strain at the permissive temperature. The mutants were found to contain higher amounts of chromosomal DNA per mg protein than the wild-type, whether or not they harbor the plasmid. Experimental results suggest that the higher amount of chromosomal DNA is due to a higher copy number of chromosomes and not to a larger amount of DNA per chromosome. These properties in each of the mutants are caused by a single mutation at the rpoB or rpoC gene that code for the beta or beta' subunit of RNA polymerase, respectively. The mutations are thought to affect the regulation of replication of oriC-bearing replicons, that is, the E. coli chromosome and oriC plasmids, but not the miniF plasmid.

MeSH Terms
Bacterial Proteins/biosynthesis Chromosome Mapping Chromosomes, Bacterial DNA Replication DNA, Bacterial/biosynthesis Escherichia coli/genetics,metabolism Gene Amplification Genetic Complementation Test Mutation Plasmids Replicon
Chemicals
Bacterial Proteins DNA, Bacterial
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tanaka M
Ohmori H
Hiraga S
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40 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00327646
Published
1983-00-00
Pages
51-60
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00327646
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