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PMID: 6360192 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Routine immunofluorescent and histochemical analysis of bone marrow involvement of lymphoma/leukaemia: the use of cryostat sections.

British journal of cancer ·Vol. 48 ·No. 6 ·1983-12-00 ·Pages 763-775

Chilosi M, Pizzolo G, Fiore-Donati L, Bofill M, Janossy G

Abstract

Enzyme histochemical and immunohistological (immuno-fluorescence and -peroxidase) techniques have been routinely used for investigating over 70 normal and pathological bone marrow samples. This recently standardized diagnostic procedure is very quick and can be performed in a few hours. In 6 cases the clinical diagnosis of leukaemia/lymphoma has become apparent only after the immunohistological analysis of the bone marrow. In 6 other cases the information about the staging of B cell malignancies was superior in the frozen biopsies to the paraffin embedded preparations. Amongst many other features the monoclonality of B CLL/lymphomas, the special features of B CLL infiltrates (RFA-1+, Leu-1+, HLA-DR+, SmIg+), follicular lymphoma deposits (containing follicular dendritic cells) and non-T, non-B acute lymphoblastic leukaemic blasts (terminal transferase+, HLA-DR+) as well as the sometimes conspicuous presence of infiltrating normal T cells could be clearly and reproducibly demonstrated.

MeSH Terms
Adolescent Adult Bone Marrow/pathology Bone Marrow Diseases/pathology Bone Marrow Examination Child Child, Preschool Fluorescent Antibody Technique Frozen Sections Histocytochemistry Hodgkin Disease/pathology Humans Immunoenzyme Techniques Leukemia/pathology Leukemia, Lymphoid/pathology Lymphoma/pathology Microtomy
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Chilosi M
Pizzolo G
Fiore-Donati L
Bofill M
Janossy G
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39 references, click to expand
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Article Info
Journal
British journal of cancer
Abbr.
Br J Cancer
ISSN
0007-0920
DOI
10.1038/bjc.1983.265
Published
1983-12-00
Pages
763-775
Language
English
Region
England
NLM ID
0370635
PMCID
PMC2011562
Subset
IM
External Links
PubMed source DOI: 10.1038/bjc.1983.265 PMC Full Text View journal details
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