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PMID: 6362894 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Stabilization of the maturation promoting factor (MPF) from Xenopus laevis oocytes. Protection against calcium ions.

Cell differentiation ·Vol. 13 ·No. 2 ·1983-10-00 ·Pages 143-8

Hermann J, Bellé R, Tso J, Ozon R

Abstract

Maturation promoting factor (MPF) activity was recovered from progesterone-matured Xenopus oocytes cytosol, fractionated by polyethylene glycol-20,000 or ethanolic precipitation. An improved stabilization of the biological activity was obtained by adding adenosine 5'-O-(3-thiotriphosphate) (ATP-gamma-S) (50 microM) to the preparation buffers. Neither Ca2+ ions nor calmodulin inhibit the partially purified MPF.

MeSH Terms

Adenosine Triphosphate/analogs & derivatives,pharmacology Animals Buffers Calcium/pharmacology Female Growth Substances/isolation & purification Maturation-Promoting Factor Meiosis/drug effects Oocytes/analysis Polyethylene Glycols Protease Inhibitors Protein Denaturation/drug effects Xenopus laevis

Chemicals

Buffers Growth Substances Protease Inhibitors Polyethylene Glycols Adenosine Triphosphate Maturation-Promoting Factor Calcium

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Hermann J

Bellé R

Tso J

Ozon R

Article Info

Journal

Cell differentiation

Abbr.

Cell Differ

ISSN

0045-6039

DOI

10.1016/0045-6039(83)90106-9

Published

1983-10-00

Pages

143-8

Language

English

Region

Ireland

NLM ID

0342640

Subset

External Links

PubMed source DOI: 10.1016/0045-6039(83)90106-9

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Stabilization of the maturation promoting factor (MPF) from Xenopus laevis oocytes. Protection against calcium ions.

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