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PMID: 6363385 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Repression of nitrogen fixation in Klebsiella pneumoniae at high temperature.

Journal of bacteriology ·Vol. 157 ·No. 2 ·1984-02-00 ·Pages 460-4

Brooks SJ, Collins JJ, Brill WJ

Abstract

The effect of elevated growth temperature on nif mRNA synthesis, nif protein synthesis, and nitrogenase activity was investigated in wild-type and NifL- strains of Klebsiella pneumoniae. Nitrogenase activity is not affected at 41 degrees C; however, nitrogenase is not synthesized at that temperature. Transcription of three nif operons studied is repressed at 41 degrees C. We show that the nifL protein is not required for repression by high temperature and propose that repression of nif at 41 degrees C results from a reversible inactivation of the nifA gene product.

MeSH Terms
Acetylene/metabolism Enzyme Repression Hot Temperature Klebsiella pneumoniae/enzymology,genetics Mutation Nitrogen Fixation Nitrogenase/genetics
Chemicals
Nitrogenase Acetylene
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Brooks S J
Collins J J
Brill W J
References (28)
28 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.157.2.460-464.1984
Published
1984-02-00
Pages
460-4
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215270
Subset
IM
Grants
NIGMS NIH HHS · GM22130 · United States
External Links
PubMed source DOI: 10.1128/jb.157.2.460-464.1984 PMC Full Text View journal details
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